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Relationship Between VEGF mRNA and Secreted VEGF Levels in ALK+ vs ALK- Cells

This study investigates the relative amounts of VEGF mRNA and the levels of secreted VEGF in different cell lines, including ALK-positive and ALK-negative variants. The data reveals distinct expression profiles associated with the presence of these genetic markers, highlighting their potential implications in lymphoma and other malignancies. Using quantitative measurements, we analyzed several ALCL cell lines (e.g., ALCL-10, ALCL-22) and correlated VEGF expression with methylation status of specific genes including DLEU2 and SMC4.

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Relationship Between VEGF mRNA and Secreted VEGF Levels in ALK+ vs ALK- Cells

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  1. A B * 10.0 7.5 * Relative amount of VEGF mRNA 5.0 2.5 0.0 ALK n=4 + n=4 - n=4 + n=4 - n=4 tTA ALK+ no Dox ALK- With Dox * * tTA NPM-ALK TPM3-ALK C 40 30 Amount of secreted VEGF (pg/ml) 20 10 0 + n=5 - n=5 + n=5 - n=5 ALK n=6 tTA NPM-ALK TPM3-ALK Dejean_FigS1

  2. ALCL-10 ALCL-21 ALCL-22 ALCL-25 ALCL-27 ALCL-29 ALCL-32 ALCL-33 ALCL-35 ALCL-37 ALCL-38 ALCL-39 ALCL-40 ALCL-53 H20 UC MC Methylated DLEU2 (176bp) hsa-miR-16-1 Non-methylated DLEU2 (181 bp) Methylated SMC4 (240 bp) (1) Non-methylated SMC4 (238 bp) (1) hsa-miR-16-2 Methylated SMC4 (169bp) (2) 1000 ** 750 Relative miR-16 expression Non-methylated SMC4 (171 bp) (2) 500 250 0 Sc miR-16 Dejean_FigS2 Dejean_FigS3

  3. ALCL-22 ALCL-27 ALCL-39 ALCL-33 Dejean_FigS4

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