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Advanced 3D Surface Reconstruction from SEM Images of Microscopic Objects

This research addresses the challenging problem of restoring 3D surface models of microscopic objects from 2D images obtained through Scanning Electron Microscopy (SEM). By applying computer vision techniques and differential evolution algorithms, we optimize the geometric and positional parameters to generate realistic 3D models. The study includes detailed methods for visualizing structures such as Tapetal cells and Diatoms, allowing for rotation and depth interpretation. Our objective is to enhance 3D technology for microscopic analysis, providing valuable insights into biological structures.

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Advanced 3D Surface Reconstruction from SEM Images of Microscopic Objects

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  1. Ahmad Pahlavan Tafti a, Zeyun Yu a, Andrew Kirkpatrick b, Heather A. Owen b aDepartment of Computer Science, University of Wisconsin Milwaukee b Department of Biological Sciences, University of Wisconsin Milwaukee SEM 3D Surface Reconstruction Problem Statement Restoring back the 3D surface model of a microscopic object is extremely difficult to solve while its three dimensional shape in a real world is only projected into 2D digital images using a Scanning Electron Microscope (SEM). Computer vision exposes a great ability to restore the geometry of the scene by solving the inversion problem going from 2D to 3D. Objectives To bring 3D technology for microscopic objects. To create realistic anatomic shape from microscopic objects. To allow rotation and depth for further interpretation of microscopic objects.

  2. We normally initialize the 3D points and relative poses with some error thresholds. The latter process is a minimization routine that optimizes the 3D geometry information and rotation/translation parameters together. Algorithm and Approach • We took Differential Evolution into account to solve this optimization problem.

  3. Input Output 3D Visualization Going from 2D to 3D We generated a 3D model of a Tapetal cell using only its 2D images. We solved inverse problem going from 2D to 3D. We took multiple 2D images from a Tapetal cell using Scanning Electron Microscope (SEM). Going from 2D to 3D We generated a 3D model of a Diatom using only its 2D images. We solved inverse problem going from 2D to 3D. We took multiple 2D images from a Diatom using Scanning Electron Microscope (SEM).

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