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Arabidopsis Gene Project

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Arabidopsis Gene Project

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    1. Arabidopsis Gene Project GK-12 April Workshop Karolyn Giang and Dr. Mulligan Explain that I’m going to lead them thru the project. Explain Reverse, explain cDNA, print out slides for teachersExplain that I’m going to lead them thru the project. Explain Reverse, explain cDNA, print out slides for teachers

    2. Reverse genetics An approach used to determine the function of a gene from known DNA sequence

    3. Blast is a program that allows you to search for regions of local similarity between your sequence of interest and all other sequences submitted to NCBIBlast is a program that allows you to search for regions of local similarity between your sequence of interest and all other sequences submitted to NCBI

    4. Gene project Since you have a nucleotide sequence..Since you have a nucleotide sequence..

    5. NCBI homepage

    6. 1. What is the name of the gene that the unknown cDNA sequence is derived from?

    7. 1. What is the name of the gene that the unknown cDNA sequence is derived from?

    8. 1. What is the name of the gene that the unknown cDNA sequence is derived from? Explain E value. If you click on the accession number, it’ll take you to…Explain E value. If you click on the accession number, it’ll take you to…

    9. 1. What is the name of the gene that the unknown cDNA sequence is derived from? Explain E value. If you click on the accession number, it’ll take you to…Explain E value. If you click on the accession number, it’ll take you to…

    10. 2. Identify the location of this gene by Arabidopsis thaliana chromosome and genome locus.

    11. What is the function of this gene?

    12. 4. How many nucleotides are in the coding sequence of this gene?

    13. 4. How many nucleotides are in the coding sequence of this gene? Point out start and stop codonPoint out start and stop codon

    14. 5. What sequence of amino acids is encoded by your unknown cDNA sequence? If you scroll back up on the same pageIf you scroll back up on the same page

    15. 6.In the Arabidopsis genome, what other gene has the most similar protein sequence to your gene?

    18. 7. In the human genome, what is the most closely related gene to your Arabidopsis gene?

    19. 7. In the human genome, what is the most closely related gene to your Arabidopsis gene?

    20. 8. What T-DNA insertion for this gene would be likely to knock out gene function?

    21. 8. What T-DNA insertion for this gene would be likely to knock out gene function?

    22. 8. What T-DNA insertion for this gene would be likely to knock out gene function?

    23. 8. What T-DNA insertion for this gene would be likely to knock out gene function?

    24. 9.Give a literature citation to a research article that studied a knock out of this gene in Arabidopsis.

    25. 9.Give a literature citation to a research article that studied a knock out of this gene in Arabidopsis.

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