2. 3Glucose-6-P 3Ru5P + Gln F6P + E4P + R5P 3PG NH3 + 2PEP + E4P + R5P + Serine Tryptophan + pyruvate + GAP + CO2

3. Aldolase

4. 2-keto-3-deoxy-arabinoheptulosonate-7-phosphate synthase

13. chorismate synthase

14. Anthranilate Chorismate Prephenate p-hydroxybenzoate p-aminobenzoate Tryptophan Nictotinamide Phenylalanine Tyrosine Tetrahydrofolate Coenzyme Q

16. +

17. Ribose-5-phosphate Cost = 2 ATP

26. 3 glucose + 5NADP+ + 2NAD+ + 2ATP serine + glutamine ---> Tryptophan + 5NADPH + 2NADH + 2ADP + 2Pi + GAP + pyruvate + 4CO2 + glutamate glutamine costs NADPH + 2NH4+ + 2ATP GAP + glutamate + ATP + 2NADH = serine 3 glucose + 4NADP+ + 2NH4+ + 4NAD+ + ADP + Pi ---> Tryptophan + 4NADPH + 4NADH + ATP + pyruvate + 4CO2

28. Chorismate mutase

29. prephenate

30. 3 glucose + 2NAD+ + 4NADP+ + NH4+ + ATP ---> phenylalanine + R5P + 4NADPH + 2NADH + 4CO2 + ADP + Pi 3 glucose + 3NAD+ + 4NADP+ + NH4+ + ATP ---> tyrosine + R5P + 4NADPH + 3NADH + 4CO2 + ADP + Pi In animals tyrosine is made from phenylalanine by phenylalanine hydroxylase

31. Tyrosine production from phenylalanine

34. Tryptophan: indolamine-2,3-dioxygenase

35. Kynurenine N-formylkynurenine formamidase

36. N10-formyl-THF synthetase Loss of ATP! N10-formyl-THF

37. Kynurenine-3-hydroxylase (monooxygenase)

38. Kynurenine 3-hydroxykynurenine

39. Lose 1 NADPH!

40. Kynureninase

42. So…Trp = alanine - ATP - NADPH + 3-hydroxyanthranilate If: Ala = 13 ATP Then: Trp = 12 ATP - NADPH + 3-hydroxyanthranilate

43. 2-amino-3-carboxymuconate-6-semialdehyde 3-hydroxyanthranilate dioxygenase

44. If you need nictotinamide it ends here

45. Assume loss of NADH

47. + NH3 Trp = 12 ATP - 2 NADPH - (NADH?) + NADH + -ketoadipate + NH3

48. -ketoadipate dehydrogenase Same mechanism as pyruvate dehydrogenase and -ketoglutarate dehydrogenase.

49. -ketoadipate = NADH + glutaryl-CoA

50. -ketoadipate = NADH + FADH2 + glutaconyl-CoA