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3Glucose-6-P

3Glucose-6-P. 3Ru5P. +. Gln. F6P + E4P + R5P. 3PG. NH 3. +. 2 PEP + E4P + R5P. +. Serine. Tryptophan + pyruvate + GAP + CO 2. Aldolase. 2-keto-3-deoxy-arabinoheptulosonate-7-phosphate synthase. chorismate synthase. Anthranilate. Chorismate. Prephenate. p-hydroxybenzoate.

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3Glucose-6-P

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  1. 3Glucose-6-P 3Ru5P + Gln F6P + E4P + R5P 3PG NH3 + 2PEP + E4P + R5P + Serine Tryptophan + pyruvate + GAP + CO2

  2. Aldolase

  3. 2-keto-3-deoxy-arabinoheptulosonate-7-phosphate synthase

  4. chorismate synthase

  5. Anthranilate Chorismate Prephenate p-hydroxybenzoate p-aminobenzoate Tryptophan Nictotinamide Phenylalanine Tyrosine Tetrahydrofolate Coenzyme Q

  6. +

  7. Ribose-5-phosphate Cost = 2 ATP

  8. 3 glucose + 5NADP+ + 2NAD+ + 2ATP serine + glutamine ---> Tryptophan + 5NADPH + 2NADH + 2ADP + 2Pi + GAP + pyruvate + 4CO2 + glutamate glutamine costs NADPH + 2NH4+ + 2ATP GAP + glutamate + ATP + 2NADH = serine 3 glucose + 4NADP+ + 2NH4+ + 4NAD+ + ADP + Pi ---> Tryptophan + 4NADPH + 4NADH + ATP + pyruvate + 4CO2

  9. Chorismate mutase

  10. prephenate

  11. 3 glucose + 2NAD+ + 4NADP+ + NH4+ + ATP ---> phenylalanine + R5P + 4NADPH + 2NADH + 4CO2 + ADP + Pi 3 glucose + 3NAD+ + 4NADP+ + NH4+ + ATP ---> tyrosine + R5P + 4NADPH + 3NADH + 4CO2 + ADP + Pi In animals tyrosine is made from phenylalanine by phenylalanine hydroxylase

  12. Tyrosine production from phenylalanine

  13. Tryptophan: indolamine-2,3-dioxygenase

  14. Kynurenine N-formylkynurenine formamidase

  15. N10-formyl-THF synthetase Loss of ATP! N10-formyl-THF

  16. Kynurenine-3-hydroxylase (monooxygenase)

  17. Kynurenine 3-hydroxykynurenine

  18. Lose 1 NADPH!

  19. Kynureninase

  20. So…Trp = alanine - ATP - NADPH + 3-hydroxyanthranilate If: Ala = 13 ATP Then: Trp = 12 ATP - NADPH + 3-hydroxyanthranilate

  21. 2-amino-3-carboxymuconate-6-semialdehyde 3-hydroxyanthranilate dioxygenase

  22. If you need nictotinamide it ends here

  23. Assume loss of NADH

  24. + NH3 Trp = 12 ATP - 2 NADPH - (NADH?) + NADH + -ketoadipate + NH3

  25. -ketoadipate dehydrogenase Same mechanism as pyruvate dehydrogenase and -ketoglutarate dehydrogenase.

  26. -ketoadipate = NADH + glutaryl-CoA

  27. -ketoadipate = NADH + FADH2 + glutaconyl-CoA

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