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Lab Notebook Format

Lab Notebook Format. 1) Title/Date 2) Pre-lab (Parts A, B, C) 3) Purposes (2-3) 4) Personal Account 5) Discussion– only 1 question (on bottom of pg 3 of packet) 6) Conclusion. Lab 2: Enzyme catalysis Guaiacol + 2H 2 O 2 tetraguaiacol + 8H 2 O

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Lab Notebook Format

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  1. Lab Notebook Format • 1) Title/Date • 2) Pre-lab (Parts A, B, C) • 3) Purposes (2-3) • 4) Personal Account • 5) Discussion– only 1 question (on bottom of pg 3 of packet) • 6) Conclusion

  2. Lab 2: Enzyme catalysis Guaiacol + 2H2O2 tetraguaiacol + 8H2O (colorless) (brownish orange) Enzyme = peroxidase Substrate = peroxide (H2O2) How will we measure enzyme function? - Color change using a spectrophotometer peroxidase

  3. How does a spectrophotometer work? • Take home points • Light at a specific wavelength is sent through a liquid sample • Light passing through the sample is detected on the other side • To measure this accurately, we must calibrate (blank) the spec. • Calibrating the spec sets the range & “tells” the spec: • what 0% light is (darkness) • what 100% light is (all light passing through) • For our experiment we will see how the amount of light passing through changes as the reaction proceeds.

  4. Set wavelength to 500 nm • With the chamber empty, use left knob to adjust to 0% T • Insert tube #1 (blank) into spec & use right knob to adjust to 100% T • Pour #2 into #3 & begin timing immediately!! • Invert 2X quickly & place into spec • Record %T every 10 sec for 120 secs • From page 3, choose your experiment for tomorrow

  5. Enzyme Lab Overview • Only hypothesis/procedure/data from THURSDAY needs to be included. • Slope calculation for EACH of your four lines • ΔY / ΔX (Units = Y units / X units) • Discussion section (section #5) is only ONE question! • Math Overview • Be sure you’re clear on how to solve the first four problems on the Unit 3 Math Practice sheet!

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