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This study conducted by Marius Nicolae and Ion Măndoiu at the University of Connecticut presents an empirical comparison of various sequencing protocols used for profiling gene and isoform expression. The paper evaluates the performance of different techniques in terms of sensitivity, accuracy, and reproducibility. Through extensive experimental analysis, the authors aim to identify the most effective protocols for transcriptomic studies, aiding researchers in selecting appropriate methodologies for their genomic research and improving the reliability of expression data.
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Empirical Comparison of Protocols for Sequencing-Based Gene and IsoformExpression Profiling Marius Nicolae and Ion Măndoiu (University of Connecticut)