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1) genomic DNA

P. P. C TGCA G. C TGCA G. G ACGT C. G ACGT C. (Mix). 1. :. 1. :. 0.5. 1) genomic DNA. 2) Pst I digestion. 3) Adaptor ligation. (adaptor). (genomic DNA). PT1 adaptor. biotinylated- adaptor. sample A (reference). ACGT. PT2 adaptor. sample B. ACGT. PT3 adaptor.

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1) genomic DNA

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  1. P P C TGCA G C TGCA G G ACGT C G ACGT C (Mix) 1 : 1 : 0.5 1) genomic DNA 2) Pst I digestion 3) Adaptor ligation (adaptor) (genomic DNA) PT1 adaptor biotinylated- adaptor sample A (reference) ACGT PT2 adaptor sample B ACGT PT3 adaptor sample C (reference) ACGT 4) Mixture each sample & Mbo I digestion M GATC GATC GATC 5) Purification with the magnetic beads coated with streptavidin & PCR amplification Dynabeads- streptavidin locus-specific primer fluorescent dye-labeled adaptor primer 6) Measurement of concentration of the PCR product PT2 PT1 PT3 fluorescent intensity length (bp)

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