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Protein and Lipid Analysis in WT and nyc1/nol Mutant Seeds: Insights into Storage Variability

This study investigates the protein and lipid contents in both wild-type (WT) seeds and the nyc1/nol mutant seeds, focusing on storage time effects. Total protein was quantified from 10 dry seeds, revealing variability indicated by standard deviation bars. Lipid extraction and quantification were carried out using 10-50 mg of seeds, and results were similarly depicted. SDS-PAGE analysis provided insights into total protein separation, while TLC was utilized for lipid visualization, showcasing differences in composition between fresh and aged seeds.

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Protein and Lipid Analysis in WT and nyc1/nol Mutant Seeds: Insights into Storage Variability

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  1. A B 0 35 0 54 0 35 0 54 WT nyc1/nol WT nyc1/nol Months of storage Months of storage C D kD TAG 250 150 100 75 50 37 Oxidized TAG 25 20 15 M 0 35 0 54 nyc1/nol WT 0 35 0 54 Months of storage nyc1/nol WT Months of storage Supplemental Figure S2. Protein and lipid contents in WT seeds and nyc1/nol mutant seeds (fresh and old seeds). A, Protein contents of seeds. Total protein was extracted from 10 dry seeds and quantified. The error bars represent the S. D. of three samples. B, Lipid contents of seeds. Total lipid was extracted from 10 to 50 mg of seeds and quantified. The error bars represent the S. D. of three samples. C, SDS-PAGE analysis of total protein of seeds. Total protein was extracted from 50 seeds with 200 μL buffer, and 10 μL of the extract was used for the analysis of SDS-PAGE. Gel was stained with CBB. D, TLC analysis of total lipid of seeds. Total lipid extracted as described in (B) was separated on a silica TLC plate developed with hexane:diethylether:acetic acid (60:40:1, v/v). Lipids were visualized by exposure to sulfuric acid.

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