1 / 26

CYTOKINE PRODUCTION OF B CELL S OF MYASTHENIA GRAVIS PATIENTS

CYTOKINE PRODUCTION OF B CELL S OF MYASTHENIA GRAVIS PATIENTS. V. Yilmaz 1 , P. Oflazer 2 , F. Aysal 3 , Y.G. Parman 2 , H. Direskeneli 4 , F. Deymeer 2 , G. Saruhan-Direskeneli 1 I.U. Istanbul Medical Faculty Departments of Physiology and Neurology,

mahala
Télécharger la présentation

CYTOKINE PRODUCTION OF B CELL S OF MYASTHENIA GRAVIS PATIENTS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. CYTOKINE PRODUCTION OF B CELLS OF MYASTHENIA GRAVIS PATIENTS V. Yilmaz1, P. Oflazer2, F. Aysal3, Y.G. Parman2, H. Direskeneli4, F. Deymeer2, G. Saruhan-Direskeneli1 I.U. Istanbul Medical Faculty Departments of Physiology and Neurology, (3)Bakirkoy Hospital (4) Marmara University, Faculty of Medicine, Division of Rheumatology, Istanbul/Turkey

  2. Neuromuscular junction Vincent A ,

  3. Auto-antibodies in MG • Anti-AChRantibody(AP) 85-90 % IgG1-2a (Complementactivated) • Seronegative MG 15-10% • Anti-AChR (-) Anti-MuSK (-) (SN) 56% 5-8% • Anti-MuSK (Musclespecifickinase) (MP) 44% 4-6% IgG4 and IgG1 (Complementactivity?)

  4. Role of Cytokines in Regulation of AntibodyProduction • T cellhelp • CD40L (Ligand) interaction • Cytokine

  5. Basal Cytokine Secretion of PBMC in MG IFNg IL-10 IL-13 * p<0.001 * p< 0.001 N 59 22 MG CON N 59 22 MG CON N 59 22 MG CON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC)

  6. CD3 Stimulation of MG PBMC IL-10 IFNg IL-13 *p<0.001 *p=0.001 N 25 16 18 SN MP AP N 25 16 18 SN MP AP N 25 16 18 SN MP AP

  7. StudyGroup

  8. B Cells in thePeriphery % of CD19+CD27+ SUBGROUPS of MG

  9. BAFF RECEPTOR (BAFFR) in MG p: 0.006 p: 0.001 SUBGROUPS of MG

  10. T HelperCells (Th2): CD4+CCR4+ p: 0.015 p: 0.04

  11. Role of Cytokines in Regulation of AntibodyProduction • T cellhelp • CD40L (Ligand) interaction • Cytokine

  12. T CellHelp: CD40L expression in isolated CD4+ T cells CON BOTH MG and CON were higher than RA

  13. T CellHelp: IL-10 and IFN-gexpression in isolated CD4+ T cells CON CON

  14. IL-10 and IL-6 expression in isolated CD19+ B cells CON CON

  15. B cell activation • B cellreceptor (BCR = Ig) SpecificantigenAChR/MuSK ImmunoglobulinPolyIg • T cellhelp CD40- CD40L • B cellco-receptors TLR TLR-9 CpG/non-CpG

  16. TLR Stimulation of B Cells

  17. Regulatory Role of B Cells by IL-10 Secretion

  18. IL-10 ResponsetoNonspecificStimuli Basal: SN<CON* MP<CON* RA<CON* AP<CON Poly Ig: SN<CON* RA<CON* MP<CON AP<CON Basal Poly Ig CpG

  19. IL-10 production of B cells in response to specific antigens AChR: MP<CON* RA<CON* SN<CON AP<CON Basal tAChR rMuSK No subgroup related antigen specific responses

  20. IL-6 ResponsetoNon-specificStimuli Basal: MP<CON* RA<CON* AP<CON SN<CON Poly Ig: MP<CON* AP<CON* RA<CON* SN<CON Basal Poly Ig CpG

  21. IL-6 Response to Specific Stimuli AChR MP < CON* RA < CON* No subgroup related antigen specific responses

  22. TNF-aResponsetoStimulation Poly Ig: MP<CON* SN<CON* Basal Poly Ig CpG No differences in responses to AChR and MuSK between groups

  23. TNF-bResponsetoNon-specificStimuli Basal: MP<CON* AP<CON* RA<CON* Poly Ig: MP<CON* SN>CON Basal Poly Ig CpG

  24. TNF-bResponseto SpecificStimuli AChR: RA<CON* No subgroup related antigen specific responses Basal tAChR rMuSK

  25. IL-12p40 Secretion in ResponsetoNon-specificStimulation AP MP SN RA CON No differences in responses to AChR and MuSK between groups

  26. CONCLUSION • B cellrelatedor B cellderivedcytokineactivity in antibody-basedsubgroups of MG did not reveal a specificpatternfordiseasedevelopment • Thedifferencesbetween RA and MG pointtodifferentialcytokineeffects in thesediseases • Untreatedpatients in allsubgroupsareneededtoclairifythedifferentialfeatures in thisdisease.

More Related