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Comprehensive Analysis of Promoter Activities in Luc Gene Variants

This report examines various Luc gene variants, focusing on the promoter activities of pGL3 basic constructs. We assess multiple configurations, including Luc.-398/+99 and Luc.-522/-157, alongside activity measurements at different levels such as 24000 and 16000. The data highlights the interaction between specific promoter sequences and transcription factors like Sp1, offering insight into gene regulation mechanisms. Through a structured analysis of reported activities, we aim to provide valuable information for researchers studying gene expression and regulatory systems.

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Comprehensive Analysis of Promoter Activities in Luc Gene Variants

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  1. Luc -398/+99-KCDT11 Luc -338/+99-KCDT11 Luc -268/+99-KCDT11 Luc -156/+99-KCDT11 -37/+99-KCDT11 Luc -522/-157-D--37/+99-KCDT11 Luc -81/+99-KCDT11 D 20000 16000 12000 Luc Activity 8000 4000 -522/-157D -37/+99 -522/+99 -37/+99 -156/+99 -81/+99 0 pGL3 basic pGL3 promoter A 8 1 6 7 2 5 3 4 13 12 17 18 19 20 11 16 15 9 10 14 26 24 23 29 28 22 27 25 21 30 37 33 34 39 31 32 35 36 38 47 45 46 40 42 41 43 44 58 53 55 57 56 54 48 52 50 49 51 59 60 66 61 67 64 65 62 63 69 68 71 70 72 B Sp1- D Sp1- E Sp1- F Sp1- C Sp1-A Sp1-B * Luc -522/+99-KCDT11 Luc C 24000 20000 16000 Luc Activity 12000 8000 4000 0 -398/+99 -338/+99 -268/+99 pGL3 basic -37/+99 -522/+99 -156/+99 pGL3 promoter

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