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1. H H A A Chd5 Poster Title Poster Title Poster Title Poster Title Author One, MD, Author Two, PhD, Author Three, BS Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester 40 Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box withFill this text box with text. 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Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text. Figure 1: Levels of chromatin packing. Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box withFill this text box with text. 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Fill this text Nucleosome DNA Nucleosome Chromosome Figure 2: Chd5 is being studied for its role in gene silencing and histone, DNA, and nucleosome modification in the onset of human gliomas, neuroblastomas, as well as other processes. , for Image adapted from accessexcellence.org and wikipedia.org Results Figure 4: PCR results for amplification of Chd5 using two types of human genomic DNA [ AA2M (A) or 2-Hybrid (H) ] show no bands at ~ 3kb. Packaging Gene Silencing DNA + Histones Tumoriogenesis Nucleosome 1 - 2 3 - 4 Primers Regulation of Cellular Processes 1 kb Ladders DNA DNA Methylation y n y n y n y n DMSO ? ? ? Nucleosome Remodeling DNA Replication Future Studies Histone Modification Transcription Other Fill this text box with text. Fill this text box with text. Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. Fill this text box with text.Fill this text box with text. 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Fill this text box with text.Fill this text box with 1 kb Ladder Brain Liver Brain Liver cDNA Primers 1-2 3-4 1-2 3-4 1-2 3-4 1-2 3-4 References • Bagchi A et al. “The Quest for the 1p36 Tumor Suppressor.” American Association • for Cancer Research Journal. 68: 2551-2556. 2008. • 2. Groth A et al. “Chromatin Challenges during DNA Replication and Repair.” Cell. • 128: 721-733. • Jones PA et al. “The Epigenomics of Cancer.” Cell. 128: 683-692. 2007. • Kouzarides T. “Chromatin Modifications and Their Function.” Cell. 128: 693-705. • 2007. • 5. Marfella CG et al. “The Chd family of chromatin remodelers.” Mutation Research.. • 618: 30-40. 2007 • 6. Sambrook, Joseph, and David Russell. Molecular Cloning. 3rd ed. Cold Spring • Harbor: CSHL Press, 2001. • 7. Thompson PM et al. “CHD5, a new member of the chromodomain gene family, • is preferentially expressed in the nervous system.” Oncogene. 22: 1002-1011. 2003. Figure 6: PCR results for amplification of Chd5 using human liver or fetal brain cDNA show no bands at ~3kb or ~6kb. 1 kb Ladder Brain Liver Primer 1: Chd5 f Primer 2: Chd5(BspHI) r Primer 3: Chd5(BspHI) f Primer 4: Chd5 r Primers 1-2 3-4 1-4 None 1-2 3-4 1-4 None Chd5 ~ 6kb Figure 3: A total of 4 primers were used in the effort to amplify the Chd5 gene: primers 1 and 4 to amplify Chd5 as a whole, primers 2 and 3 to help amplify two 3kb fragments.