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Peter Werner Final portfolio May 30, 2008

Peter Werner Final portfolio May 30, 2008. Zoom project. Zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images. More zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images. Story project: Mushroom microanatomy.

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Peter Werner Final portfolio May 30, 2008

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  1. Peter WernerFinal portfolioMay 30, 2008

  2. Zoom project

  3. Zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images

  4. More zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images

  5. Story project:Mushroom microanatomy

  6. Asci, Morchella elata (morel), 40x objective, phase contrast • Zeiss Axioskop, Zeiss Axiocam CCD image

  7. Asci, Morchella elata (morel), 40x objective, dark-field • Zeiss Axioskop, Zeiss Axiocam CCD image

  8. Asci, Morchella elata (morel), 10x objective, fluorescence, green (Rhod) ex • Zeiss Axioskop, Zeiss Axiocam CCD color image

  9. Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 63x objective, bright-field • Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image

  10. Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 100x objective, fluorescence, green (TRITC) ex • Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements monochromatic image with color overlay

  11. Cheilocystidia, Clitocybe saeva (field blewitt), 63x objective, bright-field • Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image

  12. Various basidiospores: Chroomgompus vinicolor (upper left), 100x obj; Lactarius rubidus (upper right), amyloid (iodine blue) reaction with Melzer’s reagent, 63x obj; Panaeolina foenisecii (bottom), 100x obj; all bright-field. • Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color images

  13. Resolution vs Contrast:Diatom test

  14. Overview: • Gyrosigma balticum (diatom), 63x/0.80 NA objective, bright-field • Test of resolution of surface frustules on Gyrosigma • Slide by Klaus Kemp • Zeiss Axioskop, Zeiss Axiocam CCD black and white images

  15. Resolution and contrast: NA = 0.9 (condenser aperture wide open) • Image well-resolved, but somewhat lacking in contrast

  16. Resolution and contrast: NA = 0.8 (resoution of condenser matches that of objective) • Image well-resolved and with good contrast

  17. Resolution and contrast: NA = 0.4 (condenser diaphragm at 50% field of view in back focal plane) • Image poorly resolved – Airy disk size is such that individual frustules blend together and give the false image of ridges on diatom surface. • (“Bad” image)

  18. Resolution and contrast: NA = 0.05 (condenser diaphragm stopped down to smallest aperture) • Image very poorly resolved – Airy disks so large that many points in image run together and there is little resolution. • (“Bad” image)

  19. Plants

  20. Leaf cross section, Dianthus,20x objective, phase contrast • Pre-prepared slide • Olympus CKX41/31

  21. Anther cross section, Lillium (lily),4x objective, fluorescence, green (TRITC) ex, red em • Pre-prepared slide • Olympus CKX41/31

  22. Stem cross section, Zea mays (corn),20x objective, fluorescence, green (TRITC) ex, red em • Pre-prepared slide • Olympus CKX41/31

  23. Pollen, Pinus (pine),40x objective, fluorescence, green (TRITC) ex, red em • Pre-prepared slide • Olympus CKX41/31

  24. Sporangium, Polystichum munitum (sword fern), 10x objective, fluorescence, blue (FITC) ex • Olympus CKX41/31, Nikon Coolpix 4500 digital camera color image, through ocular

  25. Insects and more diatoms

  26. Aphid (unidentified species), 4x, dark-field (Ph3 ring substituted for PhL ring) • Olympus CKX41/31 • (Semi-”bad” image: air bubble and schmutz on otherwise good image)

  27. Antenna, aphid (unidentified species), 40x, fluorescence, blue (FITC) ex • Olympus CKX41/31

  28. Leg, aphid (unidentified species), 20x, phase contrast • Olympus CKX41/31

  29. Diatom test slide, 20X, bright-field • Slide made by master diatom artist, Klaus Kemp • Olympus CKX41/31

  30. Pleurosigma angulatum (diatom), from diatom test slide, 20x, phase contrast • Olympus CKX41/31

  31. Unknowns

  32. Extra creditand other overlapping stuff

  33. Make your own slide: all slides were made by me unless otherwise noted. This would constiute at least half the slides presented here. • Dark-field: Several, including two darkfield images of morel asci (in both Zoom and Story projects) and one of the aphid images. • “Bad” images: two of the diatom slides. Problems with the darkfield aphid slide are also noted.

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