動物模式核心實驗室

1. 動物模式核心實驗室 研究技術/方法 • Tumorgenesis nude mice model • Disease animal model • Gene therapy model • Drug delivery animal model

2. 蛋白質體核心實驗室 研究技術/方法 • 2D gel electrophoresis • Protein identification:Tandem mass spectrometry [ESI/Q-TOF, MALDI-Q-TOF]: protein identification • Structural proteomics:fluorescence spectrophotometry, gel filtration, site-directed mutagenesis, circular dichrorism • Recombinant protein production • Protein purification:FPLC ,MDLC • Protein-protein interaction: real-Time biomolecule interaction analysis • Protein-protein interaction:yeast two-hybrid system

3. 細胞分子生物核心實驗室 研究技術/方法 • Laser capture microdissection • Immunohistochemistry • Electron microscope • Cell culture • Western blot • Northern blot • Immunoprecipitation • Transfection: transient, stable • Electrophoretic mobility shift assay (EMSA) • ChIP Chromatin immunoprecipitation (CHIP) • Promoter activity assay • RNAi construct • Confocal microscope • Flow cytometry

4. 基因體核心實驗室 • Microarray • Real-time PCR • DNA and RNA extraction • Gene subtraction • Serial analysis of gene expression (SAGE) • Genotyping (DNA polymorphism) 研究技術/方法

5. 生物資訊暨結構生物學 核心實驗室 研究技術/方法 • Microarray data analysis • SNP data analysis • Genome bioinformatics • sequence analysis • Design of primers • Drug design • Theoretical calculation of small molecules and macromolecules • Structural identification of small molecules and macromolecules