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Design Engineering: Cell Encapsulation

Design Engineering: Cell Encapsulation. Presented by: Audrey Gonzalez and Carlos Montalvo (CCISD) Faculty mentor: Dr. Zhengdong Cheng of the Artie McFerrin Chemical Engineering Department. Core element of research being bridged into classroom project.

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Design Engineering: Cell Encapsulation

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  1. Design Engineering: Cell Encapsulation Presented by: Audrey Gonzalez and Carlos Montalvo (CCISD) Faculty mentor: Dr. Zhengdong Cheng of the Artie McFerrin Chemical Engineering Department

  2. Core element of research being bridged into classroom project Encapsulating cells of interest with therapeutic agent in a semi-permeable membrane Implant cell capsules into human Cells release therapeutic substance such as insulin for diabetics Cell encapsulation

  3. Dr. Cheng’s lab: focused on chemistry of encapsulation The Alginic acid encapsulation is then covered by the polymer PNIPAM that exhibits controlled variations of permeability Encapsulation is made from Alginic acid which is insoluble in water/organic solvents and is biocompatible

  4. Making encapsulations in the lab 1. Make a solution of calcium cholride and water and setup camera 2. Make a solution of alginic acid, water, and turgitol and place in syringe pump

  5. 3. Set up syringe pump and voltage source 4. Test electrospray and begin making encapsulations

  6. Chemistry Concepts: properties of water, solubility, chemical/physical properties and changes, density and buoyancy, atoms (TEKS 1A, 2A, 2B, 2C, 4A, 4B, 4C, 4D, 5A, 6A, (IPC 7A), 12A, 12C) Physics concepts: interpreting graphs, measurements (time, density, mass, volume, buoyant forces, grams released per unit time), semi-permeable membrane, design engineering (TEKS 1A, 2A, 2B, 2C, 2E, 3B, 4A, 4B, 4C, 4D) AP Biology concepts: Biomedicine (time release), cell encapsulation, cell processes (osmosis/diffusion, semi-permeability), biochemistry (hydrophobic/philic bilayer, chem/phys properties of membrane, chem/phys changes to encapsulation, properties of water), immune system role CONCEPTS AND TEKS COVERED IN PROJECT TEXAS ESSENTIAL KNOWLEDGE AND SKILLS

  7. Classroom project Timeline: Cumulative project (7 weeks) Actual design, build, analysis phase (2 weeks) Objective: Lab groups will be defending a cell encapsulation design to a local pharmaceutical representative using knowledge based on research of given materials and data measurements.

  8. Classroom project Proposal: A local pharmaceutical company is looking for a new way to encapsulate a drug. The actual encapsulation must be- 1. protective of the medicine inside 2. time released 3. selectively-permeable

  9. Who cares?

  10. What is the relevance? We need drugs on the market that are safe and work properly.

  11. About 176,500 people aged 20 years or younger have diabetes. As of 2005, 20.8 million people have diabetes The estimated economic cost of diabetes in 2002 was $132 billion. Of this amount, $92 billion was due to direct medical costs. www.cdc.gov/diabetes Diabetics What is the relevance?

  12. The pharmaceutical industry is a billion dollar industry that has become a necessity in our allopathic culture today. What is the relevance?

  13. Design Phase • Students will be given 5 different semi-permeable materials to test • Size restriction • A design proposal will be submitted for approval Research: • physical and chemical properties of membrane -Do they affect semi-permeability or density? (Chemistry) • Bioencapsulations/Immunosuppression of (APBiology) • Advantages and disadvantages of time released(AP bio/chemistry/physics)

  14. Measurement phase Coffee filter Before building: • Time that it takes for small measured amount of Tums to diffuse through each membrane • Using ratios decide time for an entire packet of Tums quikpak to dissolve • Based on research decide if long or short diffusion is better • Rank membranes and choose material(s) burlap fiberglass nylon Pantyhose

  15. Measurement phase Testing of encapsulation: • Mass before/after • Volume • Density: sink, float, or suspended in water • Find buoyant forces (physics) • Measure mass released per unit time (extrapolate at 2*t, 4*t, 6*t) • Selectively permeable?

  16. Analysis phase: after testing and data reviewed, defenses will begin • Groups must clearly and concisely defend their encapsulation product, design and material, to a local pharmaceutical rep

  17. This encapsulation is made out of a double layer of nylon material that is surrounding an embroidery hoop. The “therapeutic agent” that is encapsulated is a Tums quikpak. The project Time for Tums to diffuse out of encapsulation 2.14 min

  18. This encapsulation is made of a double layer of filter paper surrounding an embroidery hoop and is encapsulating a Tums quikpak. Time for Tums to diffuse out 3.53 min

  19. Rubric for grading

  20. Pre and post-test

  21. Dr. Zhengdong Cheng • Grad student Andres Mejia • Undergrad Lucas Kinard Acknowledgements

  22. Dr. Cheryl Page • Dr. Karen Butler-Purry • Dr. Robin Autenrieth • Jacque Hodge • Joy Monroe • Dr. Andy Conkey • Dr. ArunSrinivasa • Dr. Jennifer Welch • All E3 participants • Dr. Chance Lewis

  23. Joys of teaching Ma and Pa video http://www.youtube.com/watch?v=Bfq5kju627c

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