1 / 17

Cry3A transgenic, bark beetle resistant spruce

Cry3A transgenic, bark beetle resistant spruce. NAZV project QH71290 2007-2011. Spruce bark beetle (Ips typographus). Bark beetle galleries. Bacillus thuringiensis crystal protein (Cry, δ -endoTOXin). Agrobacterium interaction with the plant cell. Major problems:.

media
Télécharger la présentation

Cry3A transgenic, bark beetle resistant spruce

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cry3A transgenic, bark beetle resistant spruce NAZV project QH712902007-2011

  2. Spruce bark beetle (Ips typographus)

  3. Bark beetle galleries

  4. Bacillus thuringiensiscrystal protein (Cry, δ-endoTOXin)

  5. Agrobacterium interaction with the plant cell

  6. Major problems: • Spruce is difficult to transform • No known Bt toxin is toxic enough to bark beetle

  7. Spruce callus cultures

  8. Somatic embryogenesis induction conversion cultivation

  9. Modified Cry3A genes Cry3A orig Synt TOX-1 (602 aa)-codons Picea optimized Ndel/sTOX-1 Cdel/sTOX-1 (602 aa) Cdel/Ndel/sTOX-1 GUS

  10. Refined modification of domain II

  11. Expression vector

  12. PAGE analysis of modified Cry3A expression

  13. WESTERN detection of modified Cry3A proteins

  14. Toxicity tests on live beetles

  15. K o n e c p r e s e n t a c e

More Related