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Assessing Cellular Response in TRF1D/D and Wildtype LT-Cre Meiosis: Implications for DNA Repair

This study investigates the cellular responses in TRF1D/D and wildtype LT-Cre mouse embryonic fibroblasts (MEF) under various conditions. We analyzed the percentage of 53BP1 positive cells and TIFs across different MEF populations. Significant differences were observed in response to ATM inhibition (KU55933) and caffeine treatments. Our results indicate the critical role of TRF1 and associated proteins in DNA repair mechanisms during meiosis, highlighting their potential as therapeutic targets in genomic stability studies.

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Assessing Cellular Response in TRF1D/D and Wildtype LT-Cre Meiosis: Implications for DNA Repair

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  1. Martinez_5435_Supplementary Fig 1A-C Wildtype-LT-Cre Wildtype-LT-Cre TRF1D/D -LT-Cre TRF1D/D -LT-Cre A B p<0.0001 n=2 MEF 21 cells 100 p<0.0001 p<0.0001 100 p<0.0001 80 n=2 MEF 246 cells 80 60 Cells with > 4 TIFs (%) 60 p=0.1 53BP1 positive cells (%) p=0.04 p=0.07 n=2 MEF 195 cells 40 n=2 MEF 17 cells 40 n=2 MEF 226 cells n=2 MEF 189 cells n=2 MEF 15 cells n=2 MEF 179 cells n=2 MEF 156 cells 20 n=2 MEF 13 cells 20 0 0 NT NT ATMi (KU55933) caffeine NT ATMi (KU55933) caffeine C Wildtype-LT-Cre TRF1D/D -LT-Cre 53BP1 + Tel

  2. Martinez_5435_Supplementary Fig 2A,B

  3. Wildtype-LT-Cre TRF1D/D-LT-Cre TIN2 TRF1 TRF2+ TRF1 TRF2+ TRF1 TIN2 +TRF1 POT1+ TRF1 POT1+ TRF1 Martinez_5435_Supplementary Fig 3A-D A B Wildtype-LT-Cre TRF1D/D-LT-Cre C D

  4. Martinez_5435_Supplementary Fig 4A-C

  5. Martinez_5435_Supplementary Fig 5A,B

  6. Martinez_5435_Supplementary Fig 5C-F C D E F

  7. Martinez_5435_Supplementary Fig 6A,B

  8. Martinez_5435_Supplementary Fig 7

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