Basic Laboratory Clinic Safety Training

1. Basic Laboratory & Clinic Safety Training

2. Basic Laboratory & Clinic Safety Training

3. Basic Laboratory & Clinic Safety Training

4. Basic Laboratory & Clinic Safety Training

5. Basic Laboratory & Clinic Safety Training Please proceed to the next section of this training by clicking the blue RETURN button below.

6. Biomedical Research EnvironmentA Unique Workplace Only workplace where all four hazards exist togetherOnly workplace where all four hazards exist together

7. Environmental Health & Safety

8. Course Objectives Chemical Hazards Texas Hazard Communication Act (HazCom) Hazardous Waste Resource Conservation & Recovery Act (RCRA) & Pollution Prevention Plan (P2) Biological Hazards Bloodborne Pathogen Standard (BBP)

9. Chemical Hazards Hazard Communication

10. Texas Hazard Communication Act Hazardous Chemicals Workplace Chemical List Employee Education Program Material Safety Data Sheets Labeling Employee Rights

11. Employee Rights You have the right to: information on your exposures receive training on chemical hazards access to MSDSs receive protective equipment (free of charge) file complaints, assist inspectors, or testify against your employer

12. Hazardous Locations Laboratories Animal care facilities Hazardous waste accumulation areas Hazardous waste processing areas Housekeeping storage areas Maintenance shops Clinics Other

13. Door Postings

14. Labeling Mandated Chemical name Brief description of hazards Manufacturer name and address When transferring chemical, label new bottle

16. Material Safety Data Sheets Available to all UTHSC-H Employees Can be accessed via the internet EH&S has link from safety homepage www.uth.tmc.edu/safety Can be obtained from the manufacturer Can be obtained by calling Chemical Safety at 713-500-5832

17. Material Safety Data Sheets ANSI�s 16 section format Most useful sections 3. Hazards identification 4. First-aid measures 6. Accidental release measures 8. Exposure controls/personal protection 9. Physical and chemical properties 10. Stability and reactivity (storage)

18. Chemical Exposure Routes of Entry Inhalation Absorption Ingestion Types of exposure Acute Chronic

19. ChemWatch Over one million MSDS from over 25,000 suppliers Over 150,000 independently reviewed MSDS Over 20 different languages http://uthsc-h.chemwatchna.com/ Turn off pop-up blockers!

28. Safe Handling of Chemicals

29. Chemical Segregation Store chemicals according to category, not in alphabetical order Alphabetize within categories Store acids, bases, flammables, oxidizers, etc. in separate locations Flammables should be stored in flammable cabinets &/or explosion-proof refrigerators - not in regular refrigerators! Label all chemicals

30. Chemical Segregation

31. Common Hazardous Categories Carcinogen Flammable Liquid Flammable Solid Combustible Liquid Corrosive Explosive Irritant Organic Peroxide Oxidizer Sensitizer Water Reactive

32. Hazardous Chemical Spill Clean-Up Minor Spill: Alert people in immediate area; Avoid breathing vapors from spill; Wear protective equipment; Use appropriate spill kit; Clean spill area. Call 713-500-5832 for assistance Major Spill: Call UTPD 713-500-4357 or 911; Alert people to evacuate; Close doors to affected area; Attend to injured personnel.

33. Chemical Exposure Controls

34. Chemical Fume Hoods Procedure and equipment at least 6 inches inside hood Sash kept at proper level and lowered when not performing work Power failure - stop work, cover or close chemicals, close hood, notify supervisor Hood is not a storage area

35. Personal Protection & Hygiene Keep body covered Pants Closed toe shoes Lab coat Proper gloves - latex examination gloves are not always appropriate Eye / face protection Always wash hands before leaving lab

36. Working Safely with Nanoparticles Currently, there are no regulations regarding the use of nanoparticles in biomedical research All nanoparticle work must be reviewed and approved by the Chemical Safety Committee Specific engineering controls, personal protective equipment and standard operation procedures may be necessary depending upon research to be completed

37. Hazardous Waste Disposal

38. Hazardous Waste Disposal Storage Utilize original container or safety cans provided by EHS Label all wastes with the chemical name including components - include the word �WASTE� or �Hazardous Waste� in the description Keep waste container closed and utilize secondary containment Waste disposal Call Waste Line (713-500-5837) select option #2, Include building, room number, and number of containers ready for pickup Waste Line is checked and wastes are collected on Monday, Wednesday, and Friday

39. Waste Minimization Plan experiments to limit waste Reduce wastes through use of microscale protocols Segregate hazardous and non-hazardous chemicals Prevent orphaned chemicals through proper labeling Avoid spills and leaks Take advantage of free chemical reuse and alcohol thermometer replacement programs Get rid of old or unwanted chemicals

40. Precursor Chemicals and Equipment Texas Higher Education Coordinating Board MOU with Texas Department of Public Safety Procurement/Purchasing Responsibilities: Keep purchase records of controlled substances, precursor drugs and laboratory apparatus, including glassware EH&S Responsibilities: Prevent the sale or distribution of precursor materials and glassware Allow audits and inspections, and designate a contact person responsible for implementing security measures Researcher & Clinician Responsibilities: Report any theft or loss to the UT Police Keep accurate inventories of controlled substances and document disposal Provide secure storage for controlled substances, precursor drugs and laboratory apparatus

41. Items Regulated as Precursor Materials

42. Controlled Substance Disposal Call hazardous waste line 713-500-5837 Press 2 for chemical waste disposal, leave a message with drugs ready for pickup, contact information, building, and room number. EHS will contact the registrant and schedule the CS pickup and destruction. The registrant and UTPD must accompany EHS to the hazardous waste processing room, and witness the blending with hazardous waste. EHS will send a record of the disposal to the local DEA office. Keep records of disposal for at least three years.

43. Surplus Transfer Process Department wishing to transfer items to surplus should fill out and submit an electronic surplus transfer form. Forms are available from the EH&S webpage at http://www.uth.tmc.edu/safety/index.html. EH&S will inspect any potentially hazardous equipment within three business days, affix a green tag, and forward the surplus request to Capital Assets Management. Surplus request for items that are non hazardous such as office equipment will be forwarded to Capital Assets Management upon receipt. Please do not store equipment awaiting surplus removal in hallways or egress corridors.

44. Proper Use of Fire Extinguishers

45. High Rise Alarm Design(a high rise is 75 ft or taller) Alarm sounds on affected floor, the floor immediately above and the floor immediately below Be aware that sound might be heard faintly on other floors, near speakers, or resonating through elevator shafts If the alarm is on your floor, you�ll know it -- its really loud!

46. Biological Safety & Bloodborne Pathogen Standard

47. OSHA Bloodborne Pathogen Standard

48. Texas Department of State Health Services Bloodborne Pathogen Control

49. Bloodborne Pathogens (BBP)

50. Bloodborne Pathogens (BBP)

51. Bloodborne Pathogens (BBP)

52. Occupational Exposure

57. Summary of UTHSC-H Statistics

60. Preventive Measures Risk of exposure can be minimized or eliminated by using the following controls: Engineering controls Personal protective equipment (PPE) Administrative controls Work place practices

61. Engineering Controls Leakproof containers Use for storage & transport of bloodborne pathogen material Sharps containers Fill no greater then � full Needleless devices Use retractable syringes, self-sheathing needles Biosafety cabinet (BSC) Directional air flow High efficiency particulate air (HEPA) filtration Access control

62. Personal Protective Equipment (PPE) Face protection Goggles or safety glasses with side shields Clothing Lab coats, scrubs, disposable gowns (long pants only and no open toed shoes!) Replace immediately if contaminated & restrict to work area Gloves Replace immediately if torn Do not wear outside the lab area

63. Administrative Controls Medical surveillance TB skin test (PPD), baseline serum Immunizations Hepatitis B series Training Management of staff (SOP compliance) Background checks, security clearance

64. Good Work Place Practices Follow SOPs Use standard precautions Treat all human blood and body fluids as if known to be infectious for HIV, HBV or other potentially infectious material Survey work area Note locations of all necessary equipment, waste containers, disinfectants, soaps Establish and maintain clean and dirty zones NEVER recap needles!!

65. Good Work Place Practices (cont.)

66. Hand Hygiene

68. Proper Hand Washing

69. Where do we miss?

70. Proper Hand Washing (cont.)

71. Most Cited Sources of Needlestick Injuries

72. Needlestick/Possible Exposure Apply routine first aid immediately Clean site of injury with soap and flush with warm water for at least 15 minutes antiseptics may be used if available Flush mucous membranes with water or saline for at least 15 minutes Notify supervisor Complete First Report of Injury Used to process insurance claims, helps identify trends Seek medical attention Needlestick Hotline (24hr): 1-800-770-9206 Employee Health: 713-500-3267 Toxicological info. Highly toxic chemical ORL RAT - LD50 of 50 mg/kg IHL-RAT - LC50 of 200 ppmToxicological info. Highly toxic chemical ORL RAT - LD50 of 50 mg/kg IHL-RAT - LC50 of 200 ppm

73. Medical Surveillance Baseline Labs HIV antibody (with consent) RPR (Syphilis) Hepatitis B surface antibody HCV antibody If source is known to be Hepatitis C+, also obtain liver function & HCV RNA tests CBC with differential and platelets, chemistry profile, urine pregnancy test if source is known HIV+ and if exposed personnel chooses to utilize post-exposure prophylaxis Hepatitis B Vaccination Tuberculosis skin test, Quantiferon test

74. CDC Exposure Risk

75. TB Presentation & Isolation

76. TB Presentation & Isolation (cont.)

77. Rights In the event of a possible exposure to bloodborne pathogens, the employee is entitled to: Confidential medical evaluation and follow-up Documentation of routes of exposure Identification, documentation, testing and results of the source individual Counseling Evaluation of reported illness

78. Blood (or OPIM) Spill Clean up Clean all blood (or OPIM) spills with a 10% solution of household bleach solution or another EPA-approved disinfectant Apply the approved disinfectant to perimeter of spill, slowly proceed inwards Allow a minimum of 15 minutes of disinfectant contact time Dispose all materials used to clean up spill (e.g., towels, gloves) in a biohazard bag

79. Biohazard Door Signs Indicates a biohazard is present Identifies the infectious agent or BSL Is present on all labs classified as BSL-2 or greater Is present on transport containers Labels all places where agent is used or stored

80. Biological Waste Disposal Steam Sterilization (Autoclave) Used for treatment of solid and liquid wastes Wastes should be packaged in heat resistant bags with the opening loosely closed to allow steam to enter bags Once waste is autoclaved, place it in a white trash can with a black liner for housekeeping Perform quality control on a routine basis and record activities in the log book Only fill bags 2/3 full Only fill bags 2/3 full

81. Biological Waste Disposal Off-Site Shipments Utilize a biological waste box or reusable red tub with a red liner Close red liner by tying the bag into a single knot Securely close the box using the self locking tabs Attach a complete biological waste label to the box or tub (designate incineration only by attaching a yellow shipping label) MSB, MSE, or SONSCC please call the hazardous waste (713 500-5837) to request collection SRB, DBB, DAC, RAS please close the box or tub and place in the regional biological waste storage room

82. rDNA Adverse Event Reporting Must report any the following to EHS: Significant problems, significant research-related accidents or illnesses involving rDNA Ex: needlestick containing rDNA; escape of transgenic animal; spills of high-risk recombinant materials outside of BSC Overt exposures to RG-2, and overt or potential exposures to RG-3 organisms must be reported immediately! Non-compliance with the NIH Guidelines Upon initial report, EHS will investigate and report to NIH Office of Biotechnology Activities and Institutional Biosafety Committee, if required, within 30 days

83. Shipping Infectious and Diagnostic Substances Training required for all persons wanting to ship infectious or diagnostic substances Refresher training required every two years or as regulations change For additional help or training information please contact Biological Safety at 713-500-4193

84. Radiation Safety Training Training required for all persons working with radioactive materials Training from previous institutions MAY fulfill requirement For additional help or training information please contact Radiation Safety at 713-500-5840

85. Information Resources Environmental Health & Safety�s webpage http://www.uth.tmc.edu/safety Biological Safety Manual OSHA Bloodborne Pathogen Standard Chemical Safety Manual Texas Hazcom Standard Hazardous Waste Contingency Plan RCRA Pollution Prevention Radiation Safety Manual Important Safety Information Posting

86. Information Resources

87. Training Certificate