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The Basics of Quality Control for Insect Rearing 2015

The Basics of Quality Control for Insect Rearing 2015. Norm Leppla. University of Florida Entomology and Nematology Department. I. Introduction. Norm’s Career in Insect Rearing. University of Arizona- Insect rearing research, 2 years

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The Basics of Quality Control for Insect Rearing 2015

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  1. The Basics of Quality Control for Insect Rearing2015 Norm Leppla University of Florida Entomology and Nematology Department

  2. I. Introduction Norm’s Career in Insect Rearing • University of Arizona- Insect rearing research, 2 years • USDA, ARS- Insectary management, Florida and Texas, 17 years • USDA, APHIS- Methods development, Washington DC and international, 7 years • University of Florida- Integrated pest management and biological control, 18 years • Rearing and quality of Tamarixia radiata (Chris Kerr) • Extension IPM (http://ipm.ifas.ufl.edu)

  3. Countries of Workshop Students

  4. Quality Control Topics • Introduction- Learning how to rear quality insects • Colony Establishment and Maintenance • Monitoring Quality • Evaluation and Management • Guidelines for Quality Systems • Total Quality Management

  5. Learning How to Rear High Quality Insects • Apprentice in an insectary • Network with other professionals • Literature plus trial-and-error • Visit other insectaries • Reviews by experts • Education and training programs

  6. MSU Insect Rearing Center Frank Davis John Schneider

  7. Online courses • Classroom courses • Webinars • Mentoring http://insectdiets.com/ 2004

  8. Insectary Manager Network Anyone associated with the mass rearing of insects 65 Members http://www.entsoc.org/

  9. Insect Rearing Professionals Google Group Subscribe: (leonwesterd@gmail.com) Léon Westerd Group moderator Head of insect rearing Wageningen UR, Netherlands

  10. VI. Total Quality Management

  11. Generic Guidelines for Quality Systems • Policy, Planning and Administration • Design Assurance and Change Control • Control of Purchased Materials • Production Quality Control • User Contact and Field Performance • Corrective Action • Employees- Select, Train and Motivate

  12. Quality Control Programs • Individual Companies Customers • International Standards ISO 9000 ASTM • The Marketplace Quality Products

  13. Specifications- Requirements for a product or service Standards- The level of quality at which a specification is written

  14. Parts of an Insect Rearing System

  15. A Complete QA System

  16. II. Colony Establishment and Maintenance II. A. Purposes for Rearing InsectsII. B. Types of Insect Rearing SystemsII. C. Options for Colonizing InsectsII. D. Maintaining the Quality of Colonized Insects

  17. II. A. Purposes for Rearing Insects

  18. White witch, Thysaniaagrippina(Lepidoptera: Noctuidae

  19. II. B. Types of Insect Rearing Systems Small-Scale Rearing: Butterflies Painted Lady Monarch

  20. “Insect rearing is never a boring task; insects are always doing something interesting and pose new challenges all the time” Annie Lorie Punky Rogers Medium-Scale Rearing

  21. Mass Rearing- Medfly El Pino, Guatemala Capacity- 3.5 billion sterile male pupae per week Metapa, Mexico

  22. II. C. Options for Colonizing Insects Peter Ebling Insect Producer Database Mgr. Great Lakes Forestry Centre 1219 Queen St. East Sault Ste. Marie, ON P6A 2E5 peter.ebling@nrcan.gc.ca (705) 541-5517

  23. World-wide listing, 35 insect & 21 nematode orders • Data submitted by sources • Searchable database • Expand clients • Adopt-a-colony http://www.insect.glfc.cfs.nrcan.gc.ca

  24. Field Collecting Cabbage Loopers Trichoplusiani

  25. Continuous Improvement of Insect Rearing

  26. II. D. Maintaining the Quality of Colonized Insects • Collection of appropriate biotypes • Colonization and strain development • Rearing proficiency • Colony management (SOPs) • Select, infuse, hybridize • Strain replacement Sam 2005

  27. Soybean Looper vs. Cabbage Looper

  28. Tritrophic Rearing System Tamarixia radiata SOPs

  29. Colony Management • Maintain- Low productivity, variability, expensive • Select- High productivity, uniformity, consistency • Infuse- Short term recovery, hybridize? • Hybridize- New strain, crosses • Replace- New strain, adaptation

  30. III. Monitoring Quality III. A. Quality Control Criteria and Standardized Tests III. B. Sampling for Consistent Quality III. C. Production, Process and Product Control

  31. III. A. Quality Control Criteria and Standardized Tests • Quantity- Number of required stage • Rate of Development-Synchronization • Viability- Emergence, sex ratio • Size- Weight or measurements • Fecundity- Oviposition and egg hatch • Adult Behavior-Flight, longevity • Field Performance- Achieve purpose

  32. Standardized QC Tests • Joop van Lenteren (2003), IOBC- Guidelines for quality control of commercially produced natural enemies, 30 species • Rose Buitenhuis (2014), Grower guide to quality assurance of biocontrol products, 22 species • Custom, develop your own, site-specific

  33. Number of Cabbage Looper Pupae Per Rearing Container Shelf 1 is lowest

  34. III. B. Sampling for Consistent Quality Count = Measurement = N Mean = Sum/Number of Counts = X Variance = Sum of (N – X)2 / n-1 = 2 Standard Deviation = Square Root of 2 = SD

  35. Quality Control Charts

  36. Tamarixiaradiata Parasitism on 3rd, 4th and 5th Instar DiphorinacitriNymphs Anonymous, UF

  37. Level of T. radiata Parasitism on D. citri

  38. Level of T. radiata Parasitism on D. citri

  39. T. Radiata Sex Ratio

  40. T. radiata Hind Tibia Length

  41. III. C. Production, Process and Product Control

  42. IV. Evaluation and Management IV. A. Structured Diagnostic Procedures IV. B. Quality Control Versus Methods Improvement IV. C. Periodic Review

  43. IV. A. Structured Diagnostic Procedures

  44. IV. B. Quality Control Versus Methods Improvement Quality Control • Monitor indicator variables • Evaluate multiple variables • Troubleshoot using QC data • Conduct evaluations rapidly • Goal is to maintain stable production Methods Improvement • Conduct evaluations methodically • Test one variable at a time • Use controlled experiments • Test results in the production system • Goal is to optimize production

  45. E. F. Knipling IV. C. Periodic Review W. G. Eden

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