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Interaction Study of AP2C1 with AtMKKs in Yeast Two-Hybrid System

This study investigates the interaction between AP2C1 and various AtMKKs using a yeast two-hybrid system. We transformed the L-40 yeast strain with pGAD424-AP2C1 and pBTM-MKK constructs, analyzing lacZ reporter gene activation through β-galactosidase assays. Notably, MKK3 displayed autoactivation in this assay. The results highlight the functional interplay between AP2C1 and AtMKKs, contributing to our understanding of their roles in signaling pathways.

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Interaction Study of AP2C1 with AtMKKs in Yeast Two-Hybrid System

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  1. pBTM MKK1 MKK2 MKK3 pBTM-MKK3 MKK4 MKK5 MKK6 MKK7 MKK8 MKK9 MKK10 0,1 0,2 0,3 0,4 0,5 ß-galactosidase activity units/mg of protein 0 1 2 3 4 5 6 Suppl.Figure 1: Interaction assay of AP2C1 with AtMKKs in yeast two hybrid system. pGAD424-AP2C1 and pBTM-MKKs were transformed into the L-40 yeast strain. The activation of lacZ reporter gene was analyzed by β-galactosidase assay. MKK3 is autoactive in yeast two hybrid assay. The pBTM-MKK3 bar shows β-galactosidase activation by co-expressing pBTM-MKK3 and empty pGAD vector Suppl. Figure 1

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