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Advancements in Arabidopsis and Synechocystis Arrays for Photosynthetic Research

This research focuses on Arabidopsis and Synechocystis as model organisms for studying photosynthesis. Arabidopsis arrays enable investigations into gene function, while Synechocystis, a unicellular cyanobacterium with a fully sequenced genome, provides valuable insights through its genetic tools and varied growth conditions. The implications of using mosses like Physcomitrella in evolutionary studies are highlighted, showcasing the significance of their physiological traits analogous to flowering plants. With 20,000+ feature microarrays, this study enhances our understanding of plant biology and evolution.

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Advancements in Arabidopsis and Synechocystis Arrays for Photosynthetic Research

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  1. Arabidopsis arrays: Process replicates

  2. Arabidopsis2 arrays

  3. Synechocystis as a Paradigm for Photosynthetic Organisms. • Unicellular cyanobacterium • Both photosynthetic and heterotrophic growth • Facile gene replacement and other genetic tools • Completely sequenced genome (1996) • 3.6 Mbp genome + 7 plasmids. • 3754 genes 3264 - chromosomal 490 - plasmid • 3575 unique proteins (Aurora and Pakrasi, August, 2003)

  4. Synechocystis arrays

  5. Synechocystis array

  6. What is Physcomitrella? (Moss) Green plants first expanded their habitat onto land by the early Silurian (430 million years ago), and subsequently diverged into various lineages with different morphologies. The bryophytes and the ancestor of vascular plans diverged early in land plant evolution. Mosses are bryophytes, and their morphologies and life cycles differ significantly from those of flowering plants, to the fascination of plant evolutionary biologists. Furthermore, many of their physiological and developmental traits are probably shared with flowering plants, such as regulation by phytohormones (e.g., auxin and cytokinin) and intracellular messengers (e.g., Ca2+). Therefore, Physcomitrella provides an opportunity to analyze the actions of these substances in a simple system. • A 20,000+ feature microarray has been designed. • Contigs from ~80,000 ESTs (http://moss.nibb.ac.jp)

  7. Moss Array

  8. Moss Arrays: Dye swap

  9. Human whole genome array

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