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Insights into Acylphosphatase Function and Evolution Across Taxa

This annotation discusses the acylphosphatase (PTHR10029) enzyme, highlighting its roles in protein maturation and carbamoyltransferase activity. It emphasizes the evolutionary aspects, noting duplications in vertebrates and other organisms like insects, protozoa, and Eubacteria. Important substrate interactions include acetyl phosphate and carbamoyl phosphate. The significance of conserved residues, such as R23 and N41, in enzymatic function is also addressed. This protein is involved in maturation processes within the HypF clade, linking protein maturation to zinc ion binding without propagating to specific cellular components.

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Insights into Acylphosphatase Function and Evolution Across Taxa

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  1. Annotation

  2. Example 1: ACYLPHOSPHATASE (PTHR10029) vertebrate ACYP2 vertebrate ACYP1 duplication in vertebrate

  3. Example 1: ACYLPHOSPHATASE (PTHR10029) insect Protoza green plants Archaea

  4. Example 1: ACYLPHOSPHATASE (PTHR10029) Eubacteria duplication Archaea- Eukaryota Eubacteria

  5. Example 1: ACYLPHOSPHATASE (PTHR10029) R23 N41 PMID:9118002 (review) substrates: acetyl phosphate carbamoyl phosphate

  6. Example 1: ACYLPHOSPHATASE (PTHR10029) • HypF: hydrogenase maturation protein F • a zinc-containing protein involved in NiFe-hydrogenase maturation • acts as a carbamoyltransferase that transfers the carbamoyl moiety of carbamoyladenylate to the COOH-terminal cysteine of HypE (another hydrogenase maturation protein) [PMID:12586941].

  7. Example 1: ACYLPHOSPHATASE (PTHR10029) live demo • Molecular Function: • propagate 'acylphosphatase activity' to the Acypclade. Those (in Acyp clade) missing either one of the two conserved residues (R23 N41) are annotated to NOT 'acylphosphatase activity' (IKR, inferred from key residues). • propagate 'carboxyl- or carbamoyltransferase activity' to the hypFclade. • propagate 'zinc ion binding' to the hypFclade. • Biological Process: • propagate 'protein maturation' to the hypFclade. • propagate 'protein carbamoylation' to the hypFclade. • Did not propagate 'phosphate-containing compound metabolic process' (TAS annotations). • Cellular Component: • none propagated (annotation to mitochondrion in mouse is based on high throughput assay). • Annotation issues: • HypFis also shown to have carbamoyl-phosphate phosphatase activity [PMID:12377778]. This annotation is not in GO yet.

  8. Example 1: ACYLPHOSPHATASE (PTHR10029) N41 R23

  9. Example 1: ACYLPHOSPHATASE (PTHR10029)

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