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NCEH Biomonitoring Program and Assessing Exposure to Acrylamide

NCEH Biomonitoring Program and Assessing Exposure to Acrylamide. Thomas Sinks, Ph.D. Associate Director for Science National Center for Environmental Health Centers for Disease Control and Prevention. Biomonitoring measures environmental chemicals in human tissues like blood or urine. .

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NCEH Biomonitoring Program and Assessing Exposure to Acrylamide

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  1. NCEH Biomonitoring Program and Assessing Exposure to Acrylamide Thomas Sinks, Ph.D. Associate Director for ScienceNational Center for Environmental Health Centers for Disease Control and Prevention

  2. Biomonitoringmeasures environmental chemicals in human tissues like blood or urine. Cigarette smoke It can answer several questions… Who is exposed? How exposed are they? Does exposure cause disease? Does exposure vary across populations? Do interventions work? Lead(Pb) Methyl parathion

  3. 18 16 14 12 G. mean blood lead levels (ug/dL) 10 8 6 4 2.7 2.0 2 0 1974 1976 1978 1980 1982 1984 1986 1988 1990 1992 1994 1996 1998 2000 Year Changes in blood lead levels in U.S. children; 1976-1999 NHANES data

  4. National Report on Human Exposure to Environmental Chemicals • To provide the public, federal partners, and policy makers with U.S. population exposure levels of important environmental chemicals. • Congressionally mandated • Partnership between NCEH lab and NCHS/NHANES

  5. National Report on Human Exposure to Environmental Chemicals • 2001 Report: 27 chemicals • 2002 Report: will include more than 75 chemicals • acrylamide not included

  6. CDC will add acrylamide to the report • CDC is developing an assay for acrylamide or glycidamide adduct of the N-terminal b-chain of hemoglobin. • CDC is developing peptide-based standards and calibrators. • Assay reflects cumulative exposure over the last 3 months, precise and accurate, independent of fasting status and diurnal variation

  7. AA Methodology(Method in Development) Sample Hemoglobin Internal Standard Hemoglobin derived peptides HPLC-MS/MS Of N-terminal peptide Enzymatic digest Isolation of red blood cells Quantitation Step 1 Step 2 Step 3

  8. Critical Next Steps • Set aside whole blood for analysis • Convene a working group to assure quality of the assay. • Collaborate on health studies to relate population-based exposure data and health risks. • Identify important exposure sources Acrylamide 8 1 6 Lys 4 7 10 Glu Val 5 9 Thr Ala 3 Glu 2 Ser Pro Leu His Glu-C

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