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Biological Sciences 210 - Genetics and Molecular Biology

Biological Sciences 210 - Genetics and Molecular Biology. Fall, 2006. Sequence of the CF Gene. The 4 Nucleotides in DNA. DNA Structure. The DNA Double Helix. E.M. of Prokaryotic Genome. Electron Micrograph of Plasmids. Genes and Intergenic Regions. Chromatin Structure.

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Biological Sciences 210 - Genetics and Molecular Biology

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  1. Biological Sciences 210 - Genetics and Molecular Biology Fall, 2006

  2. Sequence of the CF Gene

  3. The 4 Nucleotides in DNA

  4. DNA Structure

  5. The DNA Double Helix

  6. E.M. of Prokaryotic Genome

  7. Electron Micrograph of Plasmids

  8. Genes and Intergenic Regions

  9. Chromatin Structure

  10. Chromatin Structure

  11. Chromatin Structure

  12. Human Karyotype

  13. Comparative Genomes

  14. Gene Structure

  15. One Strand of a Gene is the Transcribed (Template) Strand

  16. Figure 3-4, p. 60

  17. Protein Structure

  18. The Genetic Code

  19. Missense Point Mutation Wild-TypeMissense Mutant • DNA 3'-TACTTTGTCGGTCCA…-5' 3’-TACTTTGTAGGTCCA…-5' • mRNA 5'-AUGAAACAGCCAGGU…-3' 5’-AUGAAACAUCCAGGU…-3' • Protein fMet--Lys-Gln--Pro-Gly… fMet--Lys-His--Pro-Gly…

  20. Nonsense Point Mutation Wild-TypeNonsense Mutant • DNA 3'-TACTTTGTCGGTCCA…-5' 3'-TACATTGTAGGTCCA…-5' • mRNA 5'-AUGAAACAGCCAGGU…-3’ 5'-AUGUAACAUCCAGGU…-3' • Protein fMet--Lys-Gln--Pro-Gly… fMet

  21. Silent Point Mutation Wild-Type Silent Mutant • DNA 3'-TACTTTGTCGGTCCA…-5' 3'-TACTTTGTTGGTCCA…-5' • mRNA 5'-AUGAAACAGCCAGGU…-3’ 5'-AUGAAACAACCAGGU…-3' • Protein fMet--Lys-Gln--Pro-Gly… fMet--Lys-Gln--Pro-Gly…

  22. Frameshift Mutations • READING FRAME - Translation of an mRNA must start at the appropriate AUG of the mRNA and work along the mRNA in a 5' ----> 3' direction, reading the mRNA in units of 3. It must read in the exactly correct sequence of 3-base codons. • Demonstration: • THE FAT CAT ATE THE BIG RAT • HEF ATC ATA TET HEB IGR AT

  23. Frameshift Mutation • Frameshift mutations change the reading frame of the messenger RNA. • For example: Template strand of DNA • 3'-TACAGAGTGCTGCAT…-5' • 5'-AUGUCUCACGACGUA…-3' mRNA • AUG UCU CAC GAC GUA… codons • fMet -- Ser -- His -- Asp -- Val… • Through a deletion, the DNA strand loses the fifth base in the sequence above: • 3'-TACAAGTGCTGCAT…-5' • 5'-AUGUUCACGACGUA…-3' mRNA • AUG UUC ACG ACG UA… • fMet-- Phe -- Thr -- Thr…

  24. Structure of the CFTR Protein

  25. Automated DNA Sequencing Data

  26. Constructing cDNA

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