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The Metabolometer

The Metabolometer. 指導教授 : 王啟州 學生 : 鄭傑仁. Outline. Introduction Metabolometer Design Aim2 References. Introduction(1/2).

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The Metabolometer

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  1. The Metabolometer 指導教授:王啟州 學生:鄭傑仁

  2. Outline • Introduction • Metabolometer Design • Aim2 • References

  3. Introduction(1/2) • We propose first, to design NADH/NAD fluorescence device (‘Metabolometer’ suitable to buccal and oral cavity) and construct it. This concept of ‘metabolometer’ to look at the mitochondrial states of chronic and systemic diseases and to telemeter the data has never been constructed and thus tested before. Then, we will test hardware and software for durability, accuracy and optimize their performance. • 我們首先建議，設計並製作NADH/ NAD的熒光設備(‘新陳代謝儀’適用於口腔）。這一概念的‘新陳代謝儀’來看線粒體狀態和慢性全身性疾病，以及遙測數據是從來沒有建成的，因此測試之前，我們將測試的硬體和軟體的耐用性，準確性和優化其性能。

  4. Introduction(2/2) • Secondly, we also propose to test the devices’ functionality as redox states indicator with a biological model, It has been found that baker’s yeast mitochondria have the same NADH and Flavoprotein signals and metabolic behavior as do those in animal tissue, justifying our proposal to use Baker’s yeast as a model. • 其次，我們也建議測試設備的功能性，如氧化還原狀態的指標與生物模型，已經發現麵包酵母與線粒體具有相同的NADH和黃素蛋白信號和代謝行為，如同動物組織，所以，我們建議使用麵包酵母作為一個模組。

  5. Metabolometer Design(1/3) • Fig. 1. A: Circuit board construction of prototype ‘Metabolometer’ with attached identification of the circuit elements. B: Proposed surface view of the 2 cm capsule showing the two LED sources and the two filter protected detectors with string attached for retrieval. • A:電路板結構的原型‘新陳代謝儀’附加標識的電路元件。B:表面間隔2公分顯示了兩個 LED光源和兩個濾光片保護探測器。

  6. Metabolometer Design(2/3) • The electronic engineering design of Fig. 2 consists of 450 nm and 370 nm LEDs flashed alternatively for 10msec. Attached thereto at a distance of 3 mm but protected by gelatin filters, respectively at 520 nm and 450 nm, are two Si diode detectors followed by current to voltage conversion [not shown] and sample and hold detection into a one sec time constant with detection, logarithmic conversion, signal rationing and digital signal processing using wired output for the gerbil model. In the human subject model more space is available and the ratioed signals can be telemetered to a remote indicator and digital recorder. • 電路設計如圖2由二個450 nm和370 nm的波長LED交互閃爍10msec。經過濾鏡，分別在520nm和450nm，兩個矽二極體探測器之後，電流電壓轉換和採樣和保持檢測到1秒的時間常數的對數轉換，信號和信號減法比值和遙測。

  7. Metabolometer Design(3/3)

  8. References • 1. Veech,R.L., Lawson,J.W.R., Cornell,N.W. & Krebs,H.A. Cytosolic phosphorylation potential. J. Biol. Chem. 254, 6538-6547 (1979). • 2. McGee, S., Mirkovic, J., Mardirossian, V., Elackattu, A., Yu, C.C., Kabani, S., Gallagher, G., Pistey, R., Galindo, L., Badizadegan, K., Wang, Z., Dasari, R., Feld, M.S., Grillone, G. Model-based spectroscopic analysis of the oral cavity: impact of anatomy. J Biomed Opt. Nov-Dec;13(6):064034 (2008). • 3. Zheng W., Y.Wu, DL.Li, JY. Q. Autofluorescence of epitherlial tissue: single photon versus two-phootn excitations. our. Biomed. Optics 13:15410-208 (2008). • 4. Henderson, S.T. Ketone Bodies as a Therapeutic for Alzheimer’s Disease. Neurotherapeutics Jul:5(3):470-480 (2008). • 5. Studzinski, C.M., MacKay, W.A., Beckett, T.L., Henderson, S.T., Murphy, M.P. Sullivan, P.G. and Burnham, W.M. Induction of Ketosis May Improve Mitochondrial Function and Decrease Steady-State Amyloid-beta Precursor Protein (APP) Levels in the Aged Dog. Brain Res. Aug 21 1226:209-217 Epub (2008). • 6. Puchowicz, M.A., Zechel, J.L., Valerio, J., Emancipator, D.S., Xu, K., Pundik, S., LaManna, J.C., Lust, W.D. Neuroprotection in diet-induced ketotic rat brain after focal ischemia. J Cereb Blood Flow Metab. Dec;28(12):1907-16. Epub 2008 Jul 23 (2008).

  9. 7. Plunet, W.T., Streijger, F., Lam, C.K., Lee, J.H., Liu, J., Tetzlaff, W. Dietary restriction started after spinal cord injury improves functional recovery. Exp Neurol. 213:28-35 (2008). • 8. Hartman, A.L., Lyle, M., Rogawski, M.A., Gasior, M. Efficacy of the ketogenic diet in the 6-Hz seizure test. Epilepsia. Feb;49(2):334-9. Epub 2007 Dec 6 (2008). • 9. Zhang, L.T., Yao, Y.M., Lu, J.Q., Yan, X.J., Yu, Y., Sheng, ZY.Sodium butyrate prevents lethality of severe sepsis in rats. Shock. Jun;27(6):672-7 (2007). • 10. Grinberg N, Elazar S, Rosenshine I, Shpigel NY. Beta-hydroxybutyrate abrogates formation of bovine neutrophil extracellular traps and bactericidal activity against mammary pathogenic Escherichia coli. Infect Immun. 2008. 76:2802-7. • 11. Yerby, B., Deacon R., Beaulieu, V., Liang, J., Gao, J., Laurent, D. insulin-stimulated mitochondrial adenosine triphosphate synthesis is blunted in skeletal muscles of high fat fed rats. Metabolism 57[11];1584-1590 (2008). • 12. Abdul-Ghani, M.A., DeFronzo, R.A. Mitochondrial dysfunction, insulin resistance, and type 2 diabetes mellitus. Curr Diab Rep. Jun;8(3):173-8 (2008). • 13. Abdul-Ghani, M.A., Muller, F.L., Liu, Y., Chavez, A.O., Balas, B., Zuo, P., Chang, Z., Tripathy, D., Jani, R., Molina-Carrion, M., Monroy, A., Folli, F, Van Remmen, H, DeFronzo, RA Deleterious action of FA metabolites on ATP synthesis: possible link between lipotoxicity, mitochondrial dysfunction, and insulin resistance. Am J Physiol Endocrinol Metab. 295:E678-85 (2008).

  10. Thank you for your attention!

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