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This figure illustrates the arrangement of small plots in a field where legumes are planted in mixtures alongside a grass or other non-nitrogen-fixing plant. For fields utilizing legume monocultures, only two plots are necessary, as the middle plot, representing the mixture, is omitted. The non-legume plant serves as a critical reference for calculating the total nitrogen derived from biological nitrogen fixation in the legume plots, allowing for clear assessment of legume performance in nitrogen contribution.
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Figure 1. Configuration of one set of small plots in a field with legumes planted in a mixture with a grass or other non-N-fixing plant. In fields with a legume monoculture only two plots are needed, the middle plot (mixture) is not included. The non-legume plant serves as the reference for calculations of total N derived from biological N fixation in the legume.