1 / 57

DNA

DNA. Genetics & DNA. DNA: Picture 51 http://info.bio.cmu.edu/courses/03231/ProtStruc/ProtStruc.htm B-DNA: The advent of modeling. 46 Å. 12 base sequence. (1953-2003). http://molvis.sdsc.edu/pdb/dna_b_form.pdb. DNA: Size, Shape & Self Assembly

myron
Télécharger la présentation

DNA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DNA

  2. Genetics & DNA

  3. DNA: Picture 51 http://info.bio.cmu.edu/courses/03231/ProtStruc/ProtStruc.htm B-DNA: The advent of modeling 46 Å 12 base sequence (1953-2003) http://molvis.sdsc.edu/pdb/dna_b_form.pdb

  4. DNA: Size, Shape & Self Assembly http://www.umass.edu/microbio/chime/beta/pe_alpha/atlas/atlas.htm Views & Algorithms 10.85 Å 10.85 Å Several formats are commonly used but all rely on plotting atoms in 3 dimensional space; .pdb is one of the most popular.

  5. Pyrimidines and Purines

  6. Pyrimidines and Purines • In order to understand the structure and properties of DNA and RNA, we need to look at their structural components. • We begin with certain heterocyclic aromatic compounds called pyrimidines and purines.

  7. N N N N N N H Pyrimidines and Purines • Pyrimidine and purine are the names of the parent compounds of two types of nitrogen-containing heterocyclic aromatic compounds. Pyrimidine Purine

  8. O O CH3 HN HN O O NH NH Important Pyrimidines • Pyrimidines that occur in DNA are cytosine and thymine. Cytosine and uracil are the pyrimidines in RNA. NH2 HN O NH Uracil Thymine Cytosine

  9. NH2 O N N HN N NH NH H2N N N Important Purines • Adenine and guanine are the principal purines of both DNA and RNA. Adenine Guanine

  10. O O CH3 CH3 H3C N N N HN N O O N N N CH3 CH3 Caffeine Theobromine Caffeine and Theobromine • Caffeine (coffee) and theobromine (coffee and tea) are naturally occurring purines.

  11. Question • How many pyrimidines and purines in total occur in DNA and RNA? • A) 2, 2 • B) 2, 3 • C) 3, 2 • D) 4, 1

  12. Nucleosides

  13. Nucleosides • The classical structural definition is that a nucleoside is a pyrimidine or purine N-glycoside of D-ribofuranose or 2-deoxy-D-ribofuranose. • Informal use has extended this definition to apply to purine or pyrimidine N-glycosides of almost any carbohydrate. • The purine or pyrimidine part of a nucleoside is referred to as a purine or pyrimidine base.

  14. Question • Which of the following carbohydrates are classified as deoxy sugars? • A) 1 only B) 1 and 2 • C) 1 and 3 D) 1, 3, and 4

  15. O NH2 N HN N O CH N N HOCH2 HOCH2 O O HO OH HO OH Uridine and Adenosine • Uridine and adenosine are pyrimidine and purine nucleosides respectively of D-ribofuranose. N Uridine (a pyrimidine nucleoside) Adenosine (a purine nucleoside)

  16. Question • To which atom(s) of the pyrimidine is the anomeric carbon of a sugar attached? • A) 1 • B) 2 • C) 3 • D) 4

  17. Question • Which of the following bases is only found in DNA? • A) Cytosine • B) Thymine • C) Adenine • D) Uracil

  18. Nucleotides

  19. Nucleotides • Nucleotides are phosphoric acid esters of nucleosides.

  20. NH2 N N O N N OCH2 HO P O HO HO OH Adenosine 5'-Monophosphate (AMP) • Adenosine 5'-monophosphate (AMP) is also called 5'-adenylic acid.

  21. NH2 N N O N N OCH2 HO P O HO HO OH Adenosine 5'-Monophosphate (AMP) • Adenosine 5'-monophosphate (AMP) is also called 5'-adenylic acid. 5' 1' 4' 3' 2'

  22. NH2 N N O O N N OCH2 O HO P P O HO HO HO OH Adenosine Diphosphate (ADP)

  23. NH2 N N O O O N N OCH2 O HO O P P P O HO HO HO HO OH Adenosine Triphosphate (ATP)

  24. Question • The bond that joins two nucleotides together is called a • A) amide bond. • B) peptide bond. • C) phosphodiester bond. • D) hydrogen bond.

  25. ATP Stores Energy http://chemconnections.org/organic/Movies%20Org%20Flash/flash-amines-genetic%20bases/NADH-redox.swf ATP • Each step is endothermic. • Energy for each step comes from carbohydrate metabolism (glycolysis). • Reverse process is exothermic and is the source of biological energy. • DG° for hydrolysis of ATP to ADP is –35 kJ/mol ADP AMP

  26. Nucleic Acids

  27. Nucleic Acids • Nucleic “acids”, RNA and DNA, are polymeric nucleotides (polynucleotides). • The 5’ oxygen atom of one nucleotide’s saccharideis linked to the 3' oxygen of another. • Nucleotides are commonly referred to by an abbreviation of the heterocylic nitrogen bases that define them. (C, G, A, T, U)

  28. What are the bases? • A section of a polynucleotide chain.

  29. Heterocyclic Bases http://chemconnections.org/organic/Movies%20Org%20Flash/flash-amines-genetic%20bases/purine-N-hetero-ID.swf

  30. Structure and Replication of DNA:The Double Helix

  31. Composition of DNA • Erwin Chargaff (Columbia Univ.) studied DNAs from various sources and analyzed the distribution of purines and pyrimidines in them. • The distribution of the bases adenine (A), guanine (G), thymine (T), and cytosine (C) varied among species. • But the total purines (A and G) and the total pyrimidines (T and C) were always equal. • Moreover: %A = %T, and %G = %C

  32. Composition of Human DNA For example: • Adenine (A) 30.3% Thymine (T) 30.3% • Guanine (G) 19.5% Cytosine (C) 19.9% • Total purines: 49.8% Total pyrimidines: 50.1% Purine Pyrimidine

  33. Question • Estimate the guanine content of turtle DNA if adenine = 28.7% and cytosine = 21.3%. • A) 28.7% • B) 21.3% • C) 57.4% • D) 42.6%

  34. Base Pairing • Watson and Crick proposed that A and T were equal because of complementary hydrogen bonding. 2-deoxyribose 2-deoxyribose A T

  35. Base Pairing • Likewise, the amounts of G and C were equal because of complementary hydrogen bonding. 2-deoxyribose 2-deoxyribose G C

  36. Question • The base pairs present in DNA are: • A) A---C and C---T • B) A---C and G---T • C) A---U and C---C • D) A---T and G---C

  37. The DNA Duplex • Watson and Crick proposed a double-stranded structure for DNA in which a purine or pyrimidine base in one chain is hydrogen bonded to its complement in the other.

  38. DNA • Two antiparallel strands of DNA are paired by hydrogen bonds between purine and pyrimidine bases.

  39. DNA • Helical structure of DNA. The purine and pyrimidine bases are on the inside, sugars and phosphates on the outside.

  40. Question • The base pairs present in DNA are: • A) A---C and C---T • B) A---C and G---T • C) A---U and C---C • D) A---T and G---C

  41. DNA Replication • As the double helix unwinds, each strand acts as a template upon which its complement is constructed. C T A G

  42. DNA Replication A' C T G' C' A G T'

  43. DNA-Directed Protein Biosynthesis

  44. DNA and Protein Biosynthesis • According to Crick, the "central dogma" of molecular biology is: "DNA makes RNA makes protein." • Three kinds of RNA are involved.messenger RNA (mRNA)transfer RNA (tRNA)ribosomal RNA (rRNA) • There are two main stages.transcriptiontranslation

  45. Transcription • Transcription is the formation of a strand of mRNA using one of the DNA strands as a template. • The nucleotide sequence of the mRNA is complementary to the nucleotide sequence of the DNA template. • Transcription begins at the 5' end of DNA and is catalyzed by the enzyme RNA polymerase.

  46. C T A G C T A C G A A G T A G G T C A C T G T C C A G T G A C A C T T C G T A G C T A G 5' Transcription • As double-stranded DNA unwinds, a complementary strand of mRNA forms at the 5' end. 3'

  47. G A U C G A 5' C Transcription T A G C T A C • Uracil is incorporated in RNA instead of thymine. G A A G T A G G T C A C T G T C C A G T G A C A C T T C G T A G C T A 3' G

More Related