1 / 11

Temperature Effects on Neuronal Ion Channels in Cockroaches

This experiment investigates the impact of temperature on neuronal ion channels in cockroaches, focusing on the transport of Na+ and K+ ions across neural membranes and the generation of action potentials. By observing changes in neural spikes when the temperature is adjusted, the study aims to determine the thresholds at which ion channels open or come apart due to temperature variations. Procedures include cooling and heating techniques, as well as leg dissection to analyze neural discharge under different temperatures. This research provides insights into the temperature sensitivity of neural signaling in insects.

nam
Télécharger la présentation

Temperature Effects on Neuronal Ion Channels in Cockroaches

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Experiment Three: Effect of Temperature on Neurons

  2. Ion channels transport Na+ and K+ across neural membrane • Action potentials (“spikes”) are generated by sodium ion (Na+) infusion depolarizing the cell membrane and potassium ion (K+) expulsion repolarizing it.

  3. Ion channels will not open if temperature too low

  4. Ion channels and nerve membranes come apart if temperature too high

  5. Procedures: Put the cockroach in a jar of ice water

  6. Procedures: Cut off one of roach’s legs near the body (Put petroleum jelly on exposed wound on body and leg, return cockroach to house, leg will grow back if cockroach not full grown adult)

  7. Procedures: Place leg on cork of SpikerBox, with a bit of leg overhang

  8. Procedures: Put one electrode in the femur and the other in the coxa

  9. Exercise 1: Cool the preparation and identify temperature at which neural spikes go away Note that active refridgerators can be very electrically noisy though..you may want to unplug the cold freezer You can also use a cooler of dry ice

  10. Exercise 2: Heat system and identify temperature at which neurons discharge

  11. Tabulate heat and cold limits for different legs of different species

More Related