Identification of Exon Skipping and Intron Retention Events in Non-GFP Root Protoplasts

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This study investigates exon skipping and intron retention events in root protoplasts derived from non-GFP EXP7. A total of 15,652 splice junctions (SJs) were identified, with significant findings of exon skipping events impacting over 11,000 genes. Intron retention analysis revealed 4,557 distinct events. The results provide insights into alternative splicing mechanisms in plant cells, showcasing the complexity of gene regulation. These findings contribute to our understanding of gene expression and modification in protoplasts, which are essential for genetic studies in plants.

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May 05, 2026

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Identification of Exon Skipping and Intron Retention Events in Non-GFP Root Protoplasts

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(b) (a) Exon skipping events (genes) Identified SJs (genes) in root protoplasts non-GFP EXP7 non-GFP non-GFP EXP7 EXP7 (d) (c) Identified ADA in root protoplasts 15,652 (7,141) 758 (698) 78,815 (12,794) Intron retention events (genes) 4,557 9,501 4,800 116 496 23,127 (833) (2,998) (2,310) (549) (94) (425) (e) (f) non-GFP EXP 7 non-GFP root protoplast root protoplast EXP 7 11,058/15,104 11,842/15,653 15,653/22,251 8,842/13,343 15,104/21,358 13,343/20,822 66.3% 73.2% 64.1% 75.7% 70.7% 75.7%

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