選擇性毒蕈素受體拮抗劑 -Liriodenine 之化學合成研究

1. 選擇性毒蕈素受體拮抗劑-Liriodenine之化學合成研究選擇性毒蕈素受體拮抗劑-Liriodenine之化學合成研究 • 中文摘要 • 選擇性毒蕈素受體拮抗劑-Liriodenine之化學合成研究Liriodenine (20)屬於7-oxoaporphine生物鹼，經藥理篩選發現對於carbachol誘發天竺鼠支氣管及迴腸平滑肌之收縮具有鬆弛作用，但對心臟強心作用之活性僅為平滑肌鬆弛作用之十分之一而已，表示liriodenine (20)對毒蕈素M3亞型受體具有選擇性之拮抗作用。由於liriodenine (20)於化學結構與已知選擇性M3拮抗物 (4-DAMP ) 不同而具構造特殊性。為提昇毒蕈素M3亞型受體之拮抗作用並改善其化學性狀，經化學構造修飾並探討liriodenine之化學構造與藥效關係是唯一途逕。本論文係研究liriodenine簡易且有效之化學合成方法，以期將來用於製備liriodenine類似物，做為設計支氣管擴張劑之前導藥物重要依據。 • Liriodenine之化學製備方法乃以piperonal為起始物，經與aminoacetaldehyde dimethyl acetal縮和後，利用Pomeranz-Fritsch反應製成6,7-methylenedioxyisoquinoline(26)後，再與benzoyl chloride及trimethylsilyl cyanide製成isoquinoline Reissert compound 27。與各種鄰位碘及溴取代之苯醛類進行縮合反應後，形成各種benzylisoquinoline之酯類31,32，再水解成醇類33,34，再氧化成酮類35,36衍生物，最後經由分子內環化形成liriodenine(20)；環化合成反應採用光化學、tributyltin hydride或Psychorr等三種反應，其全程合成產率各為 19 %， 17% 及 6%。其中以tributyltin hydride環化反應最為適宜未來擴量製備之用。

2. Synthesis of Liriodenine, a selective muscarinic antagonist • 英文摘要 • Synthesis of Liriodenine, A Selective Muscarinic Antagonist • Liriodenine, an 7-oxoaporphine alkaloid was found to inhibit tracheal and ileum contraction of guinea-pig induced by carbachol. It also showed to have cardiotonic effect but only one tenth potency compared with smooth muscle relaxation effect. The pharmacological activity was demonstrated that liriodenine is a selective muscarinic M3 subtype receptor antagonist. The structure of liriodenine is completely different from the known M3 antagonist (eg. 4-DAMP). To enhance the selectivity on muscarinic M3 antagonist activity, structural modification and SAR study is essential for liriodenine. In this thesis, we like to focus on the development of a new and facile synthesis of liriodenine. Application of the proposed synthetic method will be beneficial to design new lead as potential bronchodilators. • Liriodenine was prepared by condensation of piperonal and aminoacetaldehyde dimethyl acetal, and then to form the 6, 7-methylenedioxyisoquinoline (27) via Pomeranz-Fritsch reaction. Then it was reacted with benzoyl chloride and trimethylsilyl cyanide to yield isoquinoline Reissert compound 28. The Reissert 28 was condensed with o-bromo- or o-iodobenzaldehydes to form the corresponding benzylisoquinoline esters 32 and 33, and then hydrolyzed to alcohols 34 and 35 and oxidized to ketone 36 and 37. Finally, intramolecular cyclization of the alcohols 34 and 35 react either by photo irradiation or tributyltin hydride or Pschorr reaction to finish the total synthesis of liriodenine. The yields of total synthesis of liriodenine were 19% for photocyclization, 17% for organotin hydride cyclization, and 6% for Pschorr cyclization. Cyclization by tributyltin hydride is the most effective way to prepare liriodenine with high yield and it should be able to apply in large scale synthesis.