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The Effects of Blue Light at 440 nm on Potato Tuberization for Solanum tuberosum L. cv. Desiree and L. cv. Norland

The Effects of Blue Light at 440 nm on Potato Tuberization for Solanum tuberosum L. cv. Desiree and L. cv. Norland. Erin Austad 2003-04. Background. Effect of blue light on: Callus Tubers Entire Plant. Light has one of the greatest effects Plants absorb mostly blue and red light

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The Effects of Blue Light at 440 nm on Potato Tuberization for Solanum tuberosum L. cv. Desiree and L. cv. Norland

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  1. The Effects of Blue Light at 440 nm on Potato Tuberization for Solanum tuberosum L. cv. Desiree and L. cv. Norland Erin Austad 2003-04

  2. Background • Effect of blue light on: • Callus • Tubers • Entire Plant

  3. Light has one of the greatest effects • Plants absorb mostly blue and red light • Wavelength determines color of tubers • A previous experiment by Tong et al. found that Desiree formed pink tubers under blue light and white tubers in darkness • Tong et al. also found that Norlands formed no tubers under blue light and red tubers in darkness • Somatic mutations react differently and are rare

  4. Goals • Grow a somatic mutation • Find differential responses

  5. Methods: Somatic Mutations • Started with 1 Norland, 1 Red Norland and 1 Dark Red Norland • Performed subcultures • Grew plants in solid media for 2 weeks • Took 25 2-week old plants from each variety • Transferred to liquid media • Grew plants under blue light at 440 nm for 8 weeks

  6. Methods: Somatic Mutations (cont.) • Looked for: • Callus • Callus color • Tubers • Tuber color

  7. Results: Cloned Norland Plants

  8. Results: Cloned Red Norland Plants

  9. Results: Cloned Dark Red Norland Plants

  10. Conclusion: Norland, Red Norland, and Dark Red Norland Clones • Many plants produced callus as they spent more time growing • Norland and Dark Red Norland clones grew white and pink callus under blue light • Red Norland clones grew white, pink, and red callus under blue light • Cloned plants seemed to produce the same color callus as plants that were not cloned

  11. Methods: Blue Light vs. Darkness for Norland Plants

  12. Results: Blue Light vs. Darkness for Norland plants

  13. Conclusion: Blue Light vs. Darkness for Norland Plants • There was no tuberization, only callus • Callus was white on top and red near base • Tuberization will not occur under blue light for Norland potatoes • Tubers will not grow after 8-10 weeks

  14. Methods: Blue Light vs. Darkness for Desiree Plants

  15. Results: Blue Light vs. Darkness for Desiree Plants

  16. Conclusion: Blue Light vs. Darkness for Desiree Plants • All sets of plants produced tubers • Desiree plants exposed to blue light from 4-6 weeks and darkness for 4-6 weeks produced the most tubers • Tubers were white, green, and pink • Callus was mostly white and pink • Tubers grown under blue light were not ideal

  17. Further Study • Study whether or not plants are true somatic mutations • See if plants grow differently in larger test tubes or in magenta boxes • Try grafting experiment

  18. Acknowledgements • I would like to thank Dr. Cindy Tong and Katie Goeden for their wonderful support and for sharing their knowledge in the lab • I would also like to thank Ms. Fruen and Team Research for their help with my paper and project

  19. The Effects of Blue Light at 440 nm on Potato Tuberization for Solanum tuberosum L. cv. Desiree and L. cv. Norland Erin Austad 2003-04

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