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Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg

Internship: Directed evolution of Perhydrolase for improved stability in the presence of peracids and H 2 O 2. Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg Ljubica Vojcic. Techniques included in training. Molecular biology techniques:

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Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg

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  1. Internship: Directed evolution of Perhydrolase for improved stability in the presence of peracids and H2O2 Lehrstuhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg Ljubica Vojcic

  2. Techniques included in training • Molecular biology techniques: • PCR ampilification and purification of PCR product • Mutagenesis methods (epPCR, Site Saturation Mutagenesis/Site Directed Mutagenesis) • Restriction cloning of Perhydrolase gene • Megawhop cloning • Transformation of E. coli and B. subtilis strains • Biochemical methods: • Expression of Perhydrolase in B. subtilis • Screening –determination of proteolytic activity and finding improved variants for oxidative stability • Purification of Perhydrolase • Electrophoresis techniques – SDS electrophoresis and Experion system

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