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clones. mass cultures. BMhTERT-1. BMhTERT-2. BM2A2. BM2B8. BM2A7. BM2A11. BM2B2. BM2A6. BM2A10. BM10A3. BM2A9. BM10B1. BM2B12. BMEC. p16 INK4a + + + + - + + + + + + + - - - -. kDa. 23. GSTPi. 42. Actin.

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  1. clones mass cultures BMhTERT-1 BMhTERT-2 BM2A2 BM2B8 BM2A7 BM2A11 BM2B2 BM2A6 BM2A10 BM10A3 BM2A9 BM10B1 BM2B12 BMEC p16INK4a + + + + - + + + + + + + - - - - kDa 23 GSTPi 42 Actin HSCL1 54 42 Actin Supplementary Figure 1: Validation of proteomic results Immunoblot of GSTPi and HSCL1 proteins in BMECs, early and late passage BMhTERT-1 and BMhTERT-2 mass cultures, as well as 11 immortal BMhTERT clones. Actin was used as a loading control. PDs at the time the cells were assayed are indicated above the gels. Dotted lines delineate separate blots and white bars indicate cuts through the image to rearrange lanes for presentation purposes. Supplementary Figure 1

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