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Analysis of Cell Line DNA Concentrations and MTHFR Genotype Variations in Cancer Lines

This study investigates the DNA concentrations (A260/A280 ratios) of various human cancer cell lines, including HGF-1, SCC25, SCC15, and CAL27. The specific concentrations measured are 884 ng/mL for HGF-1, 895 ng/mL for SCC25, 850 ng/mL for SCC15, and 821 ng/mL for CAL27. Additionally, we explore the MTHFR C677T genotype variations, identifying specific intronic markers at -198 bp, -175 bp, and -23 bp. The study emphasizes the importance of these genetic factors in cancer research and potential therapeutic implications.

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Analysis of Cell Line DNA Concentrations and MTHFR Genotype Variations in Cancer Lines

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  1. Cell lineDNA conc.A260/A280 HGF-1 [884 ng/mL] 1.90 SCC25 [895 ng/mL] 1.87 SCC15 [850 ng/mL] 1.76 CAL27 [821 ng/mL] 1.92 MTHFR C677T Genotype B -198 bp - -175 bp - - 23 bp - HGF-1 SCC25 SCC15 CAL27 1: HGF-1 2: SCC25 3: SCC15 4: CAL27 C A GAPDH

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