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Growth Analysis of Various Yeast Strains with Different Nucleoporins and GFP Markers

This study investigates the growth characteristics of various yeast strains expressing different nucleoporins tagged with GFP. The strains are grouped based on specific nucleoporins such as Nup42, Nup116, Nup159, and Nup100, along with additional nucleoporins like Nsp1 and Nup57. Notably, these strains, including those with Los1-GFP, Sxm1-GFP, and Kap104-GFP markers, exhibit no growth, indicating potential functional interactions or dependencies on specific nucleoporins. The insights gained could elucidate the role of nucleoporins in yeast growth and intracellular transport.

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Growth Analysis of Various Yeast Strains with Different Nucleoporins and GFP Markers

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  1. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG Group 4 yeast strain + GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  2. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG Group 4 yeast strain + GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  3. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 1yeast strain + Los1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  4. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 1yeast strain + Los1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  5. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth Group 2 yeast strain + Sxm1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth no growth Nup53D Nup85D Nup170D Pom34D

  6. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth Group 2 yeast strain + Sxm1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth no growth Nup53D Nup85D Nup170D Pom34D

  7. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 3 yeast strain + Kap104-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  8. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 3 yeast strain + Kap104-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  9. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no GFP visible Group 4 yeast strain + Mtr2-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  10. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no GFP visible Group 4 yeast strain + Mtr2-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  11. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 5 yeast strain + Cse1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth no growth Nup53D Nup85D Nup170D Pom34D

  12. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth no growth Group 5 yeast strain + Cse1-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth no growth Nup53D Nup85D Nup170D Pom34D

  13. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth Group 6 yeast strain + Msn5-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  14. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no growth Group 6 yeast strain + Msn5-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  15. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG Group 7 yeast strain + Mex67-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  16. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG Group 7 yeast strain + Mex67-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  17. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no GFP visible Group 8 yeast strain + Kap114-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

  18. Wild Type Nup42DFG Nup116DFG Nup159DFG Nup100DFG Nsp1DFG Nup57DFG Nup49DFG no GFP visible Group 8 yeast strain + Kap114-GFP Nup145DFG Nup60DFG Nup1DFG Nup2DFG no growth Nup53D Nup85D Nup170D Pom34D

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