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Biochemistry of Behavior Griffith Lab

Biochemistry of Behavior Griffith Lab. Neuronal Circuits. Behavior. Molecules. ?. Biochemistry. active. inactive. Inactive. X. X. Phosphatase. Phosphatase. Active- Low. Inactive. Phosphatase. CaM. CaM. Ca 2+. T306 T307 Autophosphorylation. Active- Ca 2+ Independent. Active.

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Biochemistry of Behavior Griffith Lab

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  1. Biochemistry of BehaviorGriffith Lab

  2. Neuronal Circuits Behavior Molecules

  3. ? Biochemistry active inactive

  4. Inactive X X Phosphatase Phosphatase Active- Low Inactive Phosphatase CaM CaM Ca2+ T306 T307 Autophosphorylation Active- Ca2+ Independent Active Active Ca2+ T287 Autophosphorylation CaMKII

  5. dCASK Eag Binds and Activates CaMKII

  6. High Ca2+ Low Ca2+ ATP dCASK CaM X dCASK Ca2+ T306 T307 Autophosphorylation Inactive dCASK Refractory to CaM: Inactive Decreased pT287 CaM Bound: Active dCASK dCASK regulates CaMKII activity and autophosphorylation

  7. Cellular Mechanisms

  8. Whole-cell Patch Clamp Images: Vivian Budnik et al.

  9. Chronic Modulation of CaMKII Activity Occludes Plasticity of Intrinsic Properties

  10. Behavior

  11. ? Courtship Learning

  12. Inhibition of CaMKII- ala peptide Constitutively active CaMKII Behavior Behavior Plasticity Blocked Memory Blocked Mushroom Body Mushroom Body Central Complex Central Complex 1o Olfactory Neurons 1o Olfactory Neurons Mushroom Body Central Complex Lateral Horn Lateral Horn Antennal Glomeruli Antennal Glomeruli Lateral Horn Lateral Horn Antennal Glomeruli Genetic Dissection of the Courtship Plasticity Circuit Training Memory Enhanced Plasticity Insensitive to T287D Levels Antennal Glomeruli

  13. Neuronal Circuits Behavior Molecules Electrophysiology and Imaging Courtship Learning Signaling: CaMKII Ion Channels: Eag Scaffolds: dCASK

  14. Bassine Penthousex6-3125griffith@brandeis.edu

  15. CIin CIfi CItest X CS ( ) CS ( ) + US ( ) Classic Courtship ConditioningSiegel and Hall, 1979 test within 5 min of training 0 15 30 45 60 Time (min)

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