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Combating Biofouling

Combating Biofouling . A design experiment by Madison Evans. The Issue. source: Australian Government Dept. of Agriculture. Formation of Fouled Hulls . Polymers Bacteria Algae, worms, barnacles, mussels. Michelle Hamel. Solutions. Clean Up Scrub Power Wash Biocides. Prevention

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Combating Biofouling

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  1. Combating Biofouling A design experiment by Madison Evans

  2. The Issue source: Australian Government Dept. of Agriculture

  3. Formation of Fouled Hulls • Polymers • Bacteria • Algae, worms, barnacles, mussels Michelle Hamel

  4. Solutions Clean Up • Scrub • Power Wash • Biocides Prevention • Slick silicon coating • Copper bottom • Biocide paint • tributyltin • toxic

  5. Focus on Zebra Mussel • invasive • out-compete natives for food • sharp • damage infrastructure • Eastern Europe → Great Lakes • ballast water California Dept. of Fish and Wildlife

  6. My Solution • Mussels use a group messaging system for mating and settlement • Engineer Pelagibacter ubique • P. ubique to respond to mussel serotonin • express Grimontia hollisae collagenase gene

  7. Mussel Structure

  8. Cell Design

  9. Serotonin Receptors Collagenase

  10. Placement of Bacteria • lab culturing-72 hours • nutritious solution • dry dock growth-48 hours • spray

  11. Testing Controlled tank environments • salt • freshwater • zebra mussels • nutrients to reach reproductive age

  12. Problems • highly specific • possibility for evolution of mussels • other biofouling organisms unaddressed • difficult to implement (growth on dry boat)

  13. Alternatives • Multiple lines of bacterial spray • Universal signaling molecule of mussels instead of just serotonin

  14. Combating Invasive Zebra Mussels A design experiment by Madison Evans

  15. References Brandelli, Adriano et al. “Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers.” Electronic Journal of Biotechnology. 13 Jan 2005. <http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n1-6/428> Proal, Amy. “Understanding Biofilms.” Bacterialty: Exploring Chronic Disease. 26 May 2008. <http://bacteriality.com/2008/05/26/biofilm/> “Stopping the Mussel Invasion.” National Park Service. <http://www.nps.gov/cure/planyourvisit/mussel_facts.htm> Winner, Cherie. “Barnacles and Biofilms.” Woods Hole Oceanographic Institute. 5 Dec 2012. <http://www.whoi.edu/oceanus/feature/barnacles-and-biofilms> Reid, D.C. et al. “Biofouling in Stirred Tank Reactors: Effect of Surface Finish.” <http://books.google.com/booksid=g5miDB7M0WsC&pg=PA529&lpg=PA529&dq=do+biofilms+cause+drag& source=bl&ots=nGMKSiYnAV&sig=V4oho8Boac3GdoJfWf0w_dr6JZ8&hl=en&sa=X&ei=OL3VU7 ezIIS3yASJqoDYDA&ved=0CDIQ6AEwAw#v=onepage&q=do%20biofilms%20cause%20drag&f=false Teramura, Tamoko et al. “Cloning of a Novel Collagenase Gene from the Gram-Negative Bacterium Grimontia (Vibrio) hollisae1706B and Its Efficient Expression in Brevibacillus choshinensis.” American Society for Microbiology. 22 Apr 2011. <http://jb.asm.org/content/193/12/3049.full> Milne, Catherine T. et al. “A Comparison of Collagenase to Hydrogel Dressings in Wound Debridement.” Wounds Research. 1 Nov 2010. <http://www.woundsresearch.com/content/comparison-collagenase-hydrogel-dressings-wound-debridement> Lucas, Jared M. et al. “A molecular, morphometric and mechanical comparison of the structural elements of byssus from Mytilus edulis and Mytilus galloprovincialis.” The Journal of Experimental Biology. 5 Apr 2012. <http://jeb.biologists.org/content/205/12/1807.full.pdf> “Collagenase Guide” Sigma-Aldrich. <http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/collagenase-guide.html> Images Courtesy of the Australian Government Department of Agriculture and photographer Mila Zinkova.

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