1 / 44

اصول و کاربرد های فلوسایتومتری تحقیقاتی

اصول و کاربرد های فلوسایتومتری تحقیقاتی. دانشگاه علوم پزشکی وخدمات بهداشتی-درمانی استان قزوین. Application. What is Flow Cytometry ?. The measurement of cell in flow stream which delivers the cells in single file past a point of measurement.

nysa
Télécharger la présentation

اصول و کاربرد های فلوسایتومتری تحقیقاتی

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. s.varasteh

  2. اصول و کاربرد های فلوسایتومتری تحقیقاتی دانشگاه علوم پزشکی وخدمات بهداشتی-درمانی استان قزوین

  3. Application

  4. What is Flow Cytometry? The measurement of cell in flow stream which delivers the cells in single file past a point of measurement.

  5. Flow cytometers are engineered with precise light pathway. Light is routed through three different types of filter.

  6. Fluorescence and Fluorochromes

  7. Fluorochromes Fluorochromes absorb energy (light) and their electrons go from a ground state to an exited state. The electrons return to ground state by emitting light of lower energy , therefore longer wavelength.

  8. Example of Fluorochromes

  9. The fluorescence intensity measured is proportional to the number to the fluorescent molecules bound to the cell.

  10. Using scattered light to determine what is real!

  11. Visualizing Flow Cytometry data

  12. Flow cytometry unstained control

  13. Flow cytometry viability control

  14. Fluorescence Minus One (FMO)

  15. Compensation

  16. Compensation FITC signal is leaking into the PE detector.

  17. Gating Gate on your cell of interest

  18. When there is an obvious positive and negative population it is easy to use a control (A) to set a marker to define positivity (B).

  19. Control Negative sample (A) and test sample (B) and  (C) shows that probably all cells are weakly positive.

  20. Negative sample (A) and test sample (B). The overlay (C) shows that some cells are negative and some are positive.

More Related