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Transcription patterns and functional prediction

Transcription patterns and functional prediction. Transcription of carboxysome genes by Thiomicrospira crunogena. Thiomicrospiras inhabit an erratic habitat.

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Transcription patterns and functional prediction

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  1. Transcription patterns and functional prediction

  2. Transcription of carboxysomegenes by Thiomicrospiracrunogena

  3. Thiomicrospiras inhabit an erratic habitat JANNASCH (H.W.), WIRSEN (C.O.), NELSON (D.C.) and ROBERTSON (L.A.): Thiomicrospiracrunogena sp. nov., a colorless, sulfur-oxidizing bacterium from a deep-sea hydrothermal vent. Int. J. Syst. Bacteriol., 1985, 35, 422-424.

  4. One possible vacuum ‘mechanism’ HCO3- HCO3- HCO3- CA CO2 CO2 Ru bisco biomass

  5. Measurement steps and details • Measure gene transcription via RT-PCR • Isolate RNA from T. crunogena • High DIC • Low DIC • Set up RT-PCR reaction • More actively transcribed genes will result in a band earlier than less actively transcribed ones.

  6. RNA isolation

  7. sqRT-PCR • Set up RT-PCR • High DIC • +/- RT • Low DIC • +/- RT • Remove samples after: • 5 cycles • 10 • 15 • 20 • Et cetera

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