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Transcription Patterns and Functional Prediction of Carboxysome Genes in Thiomicrospira crunogena

This study investigates the gene transcription patterns of carboxysome genes in the sulfur-oxidizing bacterium Thiomicrospira crunogena, collected from a deep-sea hydrothermal vent. Using RT-PCR techniques, we measured the transcriptional responses under different dissolved inorganic carbon (DIC) concentrations. The findings suggest a potential mechanism for bicarbonate utilization in erratic habitats, highlighting the adaptability of T. crunogena and the functional significance of gene expression in varying environmental conditions. Detailed methodologies for RNA isolation and transcriptional analysis are included.

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Transcription Patterns and Functional Prediction of Carboxysome Genes in Thiomicrospira crunogena

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  1. Transcription patterns and functional prediction

  2. Transcription of carboxysomegenes by Thiomicrospiracrunogena

  3. Thiomicrospiras inhabit an erratic habitat JANNASCH (H.W.), WIRSEN (C.O.), NELSON (D.C.) and ROBERTSON (L.A.): Thiomicrospiracrunogena sp. nov., a colorless, sulfur-oxidizing bacterium from a deep-sea hydrothermal vent. Int. J. Syst. Bacteriol., 1985, 35, 422-424.

  4. One possible vacuum ‘mechanism’ HCO3- HCO3- HCO3- CA CO2 CO2 Ru bisco biomass

  5. Measurement steps and details • Measure gene transcription via RT-PCR • Isolate RNA from T. crunogena • High DIC • Low DIC • Set up RT-PCR reaction • More actively transcribed genes will result in a band earlier than less actively transcribed ones.

  6. RNA isolation

  7. sqRT-PCR • Set up RT-PCR • High DIC • +/- RT • Low DIC • +/- RT • Remove samples after: • 5 cycles • 10 • 15 • 20 • Et cetera

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