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mRNA and Protein Expression Analysis of AGAT, GAMT, and BB-CK in R6/2 Mice

This study investigates mRNA and protein expression levels of AGAT, GAMT, and BB-CK in the striatum and frontal cortex of 8-week-old R6/2 mice. Quantitative PCR was conducted on non-microwaved brain samples to assess mRNA levels, while Western blot analyses were performed on microwaved samples to evaluate protein expression. Results encompass data from 5 animals per group, with significance marked at p < 0.05, highlighting important insights into metabolic alterations associated with R6/2 mouse models.

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mRNA and Protein Expression Analysis of AGAT, GAMT, and BB-CK in R6/2 Mice

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  1. * * WT WT WT R6/2 R6/2 R6/2 A * WT WT WT R6/2 R6/2 R6/2 * * B Supplementary Figure: mRNA and protein expression of AGAT, GAMT and BB-CK in R6/2 mice. We analyzed the striatum and frontal cortex of 8-week old R6/2 mice. (A) Quantitative PCR were performed on non-microwaved brain samples. (B) Western blots analyses were performed on microwaved samples. n= 5 animals/group, * p<0.05.

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