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Transcript Analysis of CDKC;1 and CYCT1;4 Genes in Mutant Plants Using RT-PCR

This study conducted RT-PCR analyses to assess CDKC;1 and CYCT1;4 transcripts in mutant plants expressing RNAi constructs. The transcript levels of both genes were evaluated in mutant and transgenic plants using the Arabidopsis UBQ10 gene as a control.

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Transcript Analysis of CDKC;1 and CYCT1;4 Genes in Mutant Plants Using RT-PCR

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  1. cdkc;2-1/CDKC;1 RNAi cdkc;2-1 1 3 4 7 10 14 2 5 8 9 11 12 13 6 CDKC;1 UBQ10 cyct1;5-1/CYCT1;4 RNAi cyct1;5-1 1 3 4 7 10 14 2 5 8 9 12 6 13 11 CYCT1;4 UBQ10 Supplemental Figure 4. RT-PCR analysis of CDKC;1 and CYCT1;4 transcripts from RNAi plants. Semiquantitative RT-PCR analysis was performed to determine the transcript levels of CDKC;1 in the cdkc;2-1 mutant and the F1 mutant transfortants harboring a CDKC;1 RNAi construct (cdkc;2-1/CDKC;1 RNAi). The same RT-PCR analysis was performed to determine the transcript levels of CYCT1;4 in the cyct1;5-1 mutant and the F1 mutant transformants harboring a CYCT1;4 RNAi construct (cyct1;5-1/CYCT1;4 RNAi). The ArabidopsisUBQ10 gene was used as a positive internal control.

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