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Epifluorescence

Confocal. Epifluorescence. Zoom 1. Zoom 2. Zoom 4. x - y. x - z. 3D Deconvolution. Sassoè-Pognetto et al., J Comp Neurol 2000. Point Spread Function. Disco di Airy. Sassoè-Pognetto et al., J Comp Neurol 2000. FITC-TRITC emission. VGAT / Calb. WT. GABA A R a 1 KO.

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Epifluorescence

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  1. Confocal Epifluorescence

  2. Zoom 1 Zoom 2 Zoom 4

  3. x - y x - z 3D Deconvolution Sassoè-Pognetto et al., J Comp Neurol 2000 Point Spread Function Disco di Airy

  4. Sassoè-Pognetto et al., J Comp Neurol 2000

  5. FITC-TRITC emission

  6. VGAT/Calb WT GABAARa1 KO Wulff et al., Nat Neurosci in press

  7. a10/0 GABA parallel fiber GABA-positive terminals in the molecular layer make asymmetric synapses with Purkinje cell spines Fritschy et al., J Neurosci 2006

  8. NL2/Car8/GAD GABAAa10/0 sp sp sp NL2/mGluR1a/GAD sp NL2 does not localize ectopically at heterologous synapses with PC spines GABA Patrizi et al., PNAS 2008

  9. Schneider Gasser et al et al.,

  10. Two-photon microscopy Two photons of low energy (infrared range) can excite a fluorophore → emission of a fluorescence photon at a higher energy Excitation is restricted to a tiny focal volume (rejection of out-of-focus signals) Longer wavelengths penetrate deeper into tissue (1 mm) Lower energy photons cause relatively low damage outside the focal volume → in vivo imaging

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