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Immunology Chapter 2, Lecture 1

Immunology Chapter 2, Lecture 1. Richard L. Myers, Ph.D. Department of Biology Southwest Missouri State Temple Hall 227 Telephone: 417-836-5307 Email: rlm967f@mail.smsu.edu. Horses may be used when a large amount of antiserum is needed.

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Immunology Chapter 2, Lecture 1

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  1. ImmunologyChapter 2, Lecture 1 • Richard L. Myers, Ph.D. • Department of Biology • Southwest Missouri State • Temple Hall 227 • Telephone: 417-836-5307 • Email: rlm967f@mail.smsu.edu

  2. Horses may be used when a large amount of antiserum is needed

  3. Primates may be used in research related to vaccine development

  4. Experimental Systems • Experimental animal models include mice • easy to handle • genetically well characterized • rapid breeding cycle • Inbred strains (syngeneic) • repeated inbreeding between litter mates • C3H • C57BL/6

  5. C57 black mouse, the experimental animal of choice

  6. Comments on the use of animals in research and teaching Ethical issue?

  7. Science, 14 August 1998

  8. Adoptive-transfer systems • First the immune response of a syngeneic host is destroyed • eliminate with x-ray (650-750 rads) • then reconstitute with other lymphocytes • X-ray will lead to death of the mouse unless reconstitution is accomplished

  9. Experimental Systems • Scid mice • must be raised in a germ-free environment • can accept transplanted bone marrow cells • Cell culture systems • primary lymphoid cell cultures • cloned lymphoid cell lines • hybrid lymphoid cell lines

  10. Cell culture laboratory

  11. SCID mice grafted with human scalp and treated with X-ray to study hair loss

  12. Fetal tissue research Ethical issue?

  13. Human embryonic stem cells(see Science, vol 282, p 1061) • Pluripotential stem cells can generate all of the cells types and can self renew • Now human ES cells from the blastocyst can be grown in culture • Have been used to produce transgenic mice • With refinement in techniques we may produce directed, lineage restricted differentiation

  14. Assignment • Read Chapter 1 (Overview) for information and review only • Read Chapter 2, Experimental Systems • Review question 12 (pg 45)

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