1 / 14

بسم الله الرحمن الرحيم

بسم الله الرحمن الرحيم. GENUS: HAEMOPHILUS Prof. Khalifa Sifaw Ghenghesh. Small, Pleomorphic G-ve Rods Require Growth Factors Two factors > X & V X = haemin V = Nicotinamide adenine dinucleotide (NAD) = NAD phosphate (NADP) BA Chocolate agar.

otto-frederick
Télécharger la présentation

بسم الله الرحمن الرحيم

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. بسم الله الرحمن الرحيم GENUS: HAEMOPHILUS Prof. Khalifa Sifaw Ghenghesh

  2. Small, Pleomorphic G-ve Rods • Require Growth Factors • Two factors > X & V • X = haemin • V = Nicotinamide adenine dinucleotide (NAD) = NAD phosphate (NADP) • BA • Chocolate agar

  3. Species Growth Factor Required ---------------------------------------------- H. influenzae X + V H. parainfluenzae V H. ophrophilus X H. ducreyi X ----------------------------------------------

  4. Haemophilus influenzae • Smooth and rough colonies occur • Capsulated >> • Non-capsulated >>

  5. ANTIGENIC STRUCTURE 1. The capsular polysaccharide antigens: • 6 serotypes: a - f. • Encapsulated H. influenzae >> Quellung (capsular swelling) reaction. 2. Somatic antigens:

  6. VIRULENCE FACTORS • i. Capsule: • ii. Outer membrane components: • OMP & LOS • iii. Adherence: • iv. IgA proteases:

  7. PATHOGENESIS • Normal Carriage • H. influenzae > Human parasite • Non-capsulate HI > nasopharynx or throat of 25-80% of healthy people • Capsulate strains in 5-10% • Immunization with conjugate vaccine > • Invasive infections • Non-Invasive Disease

  8. INVASIVE INFECTIONS H. Influenzae type b Respiratory portal of entery (Inhalation) Colonization of nasopharynx Penetration of submucosa Invasion of Bloodstream Meningitis, Epiglottitis, Bacteraemia Pneumonia, Septic arthritis Non-Capsulate HI: > Meningitis and Septicaemia > the Neonate

  9. NON-INVASIVE DISEASE • Local Infections: • Physiological or Anatomical Abnormalities • Otitis Media, Sinusitis • Purulent episodes > patients with exacerbations of chronic obstructive airway disease • Non-capsulate strains of HI • Viral Infections ?

  10. LABORATORY DIAGNOSIS 1. Direct examination: • Specimen: CSF, middle ear aspirate, sputum (purulent). 2. Culture: • Chocolate agar + CO2 • Blood culture bottles • Antigenic detection • Molecular Techniques

  11. Blood agar plate culture of Haemophilus influenzae

  12. Haemophilus influenzaesatelliting around Staphylococcus aureus

  13. TREATMENT • - Systemic illness • 3rd Generation cephalosporins • TMP-SMZ, Ciprofloxacin, Clarithromycin • - Otitis media in children • - Pneumonia in adults • - Acute sinusitis • Amoxicillin, Augmentin, Clarithromycin, PREVENTION • Vaccination

  14. Haemophilus influenzae biogroup aegyptius(Haemophilus aegyptius) • Epidemic conjunctivitis • Brazillian purpuric fever • Haemophilus ducreyi> Chancroid • Haemophilus parainfluenzae

More Related