Introducing the DSQII Terry Jeffers

1. Introducing the DSQII Terry Jeffers

2. Agenda • Update on Thermo’s Environmental Initiatives • Discussion on new approaches to the challenges of 8270 • Introducing Thermo Electrons Productivity Kits • EnviroLab Forms 2.0 – High Throughput Quatification • New application note: Rapid Analysis of 8270

3. Thermo Electron: Environmental Initiative • Focus on Environmental Laboratory needs: • Requirement for Productivity • Need for good work flow in Generating, Reviewing and Reporting data • Benefit from guides for operation, maintenance and troubleshooting specific Methodologies

4. Thermo Electron: Environmental Initiative Product Sales Specialist Environmental Demo Chemist * Environmental Seed Chemist New hardware: DSQ II Productivity Kits: 8270 and 525 8260 and 524

5. Pitt Falls for Method 8270 Those little things that stop everything

6. Activity in the GC/MS system • Activity • These sites can be everywhere • Injector, column, source • SPCC compounds are used to show good system • Nitrophenols are some of the best compounds to find active sites • Minimizing Effects of Activity • Deactivated liners • Good column that shows no active sites • High flow rates to reduce the residence time of any active compound. • Balance temperature zones to reduce activity and thermal break down.

7. Chromatography How important is it really?

8. GC and Autosampler • Separations accomplished with slow chromatography • Common GC methods for 8270 • Fast injection • 250 - 300 0 C injector temperature • 1 mL/min flow rate • 30 m x 0.25 mm ID column • Oven ramps between 5 0 and 10 0 C/min from 35 0 to 300 0 C • Separations accomplished with fast chromatography • Alternate GC method for 8270 • Hot needle injection • 250 0 C injector temperature • 3 mL/min flow rate • 30 m x 0.25 mm ID x 0.5 µm column • Oven stepped ramps to provide the critical pair separations.

9. Hot Needle Injection: Run at Lower Inlet Temperature: Fewer Replacements of Liner

10. Increase the Mobile Phase: 3 mL/min vs 1mL/min • Increase capacity of stationary • No fronting of peak shape for 160 ng/uL PNA • Decrease the irreversible absorption of active species • Pentachlorophenol < 10%RSD • (5-160 ng/uL) • 0.1 ng/uL MDL • replicates at 1 ng/uL 4.7%RSD • Minimal breakdown for endrin or 4,4-DDT • Provide Gaussian shape peaks for the most volatile • No solvent skewing of NDMA and pyridine • Shorter run times: 17 minutes

11. Effects of increased flow rates • Why increase the flow rate to 3.0 mL/min • Learned from applications developed for the analysis of explosives. • Decrease the residence time of the compounds in potential active areas. • Decreases the run time without higher temperatures that could potentially damage thermally labile compounds. • Do not need to expose the column to higher temperatures that will damage it. • Provides better separations and narrower peak widths. • This is balanced with a 20:1 split flow ratio.

12. Injection: Choice of Liner

13. PNAs ( 5 ng and 160 ng)

14. NDMA and Pyridine Split Splitless

15. Two Methods: Splitless and Split

16. TRACE Ultra DSQ II GC/MS

17. Source materials and geometry • New DuraBriteTM Source Open Geometry • Passive Collector • Extends Dynamic Range • Improves Robustness • Optional Closed EI Ion Volume • Constructed of new proprietary material for enhanced inertness • Protects Lens 1 from ion burn

18. New DynaMax XRIon Detection System • SGE ETP AF635 Electron Multiplier • Detector linear range • output current >30µA • Simple snap out replacement of • multiplier assembly • New Linear/Log Hybrid Electrometer • Extends output current to 70µA • Provides dynamic range to >106 • No loss of response or precision at the lower • sample/analyte levels

19. Expected Results Results I can get, you can get them too.

20. Calibration Curve • First page of the calibration curve report • All SPCC and CCC requirements met • Average of all RSD calibration curves of 7.46%

21. SPCCs (5ng to 160 ng min >0.05)

22. CCCs < 30% RSD for Initial Calibration Curve

23. Quantitated Data File • First page of the Quantitation Report • This shows the quantitation of the 20 ng/uL sample for the calibration curve • Meets the requirements of the internal standards and surrogates • Meets all ion ratio requirements • No manual integrations were required

24. Robustness: Split (2gr sediment/2mL methylene chloride + 25 uL diesel fuel)

25. How can we prove it Productivity Solutions

26. Productivity Solutions Integrating the DSQ II with Software, Methods, and Consumables to Get Productive, Quickly

27. Productivity Solutions • Provide all of the necessary material to run the method • Proven Thermo Column • Deactivated Liners • Standards • Snap and shoot – prediluted for entire method validation • All other consumables for about 2 weeks • Provide all of the software • EnviroLab Forms 2.0 • Interactive Reference CD • All of the methods, sequences, • Validation data used to generate all of what you see here and in the manuals • Two Boxes full of materials, manuals, and CD’s.

28. Productivity Kit • SOP manual for method • How to Guide • Quick Start Guide • Provide consumables for running method • Standards, liners, septa, syringes, column,… • Instrument method, processing method, reporting package • CD deliverable • SOP • Data from method validation • Instrument method • Processing method • Reporting software with templates

29. Pieces of the Productivity Kit

30. Quick Start Guide

31. 8270C/D Productivity Solution • Customer’s system can be taken from installation sign off to method IDL’s in as little as 2 days! • 8270 is a value added product which will sell for ~ $3000 US • Add on service provided by Thermo FSE’s • US Field Service Engineers are being trained on the 8270 PS • Kits serve as a great tool for training aides for the lab

32. Productivity Solutions are a Win-Win • Good for customers – • Improves overall experience • Increases satisfaction • Eases transition to Thermo GC/MS • Gives optimal performance for the DSQ II • Good for Thermo • Easy to support these solutions Helps the customer and support by establishing a known set of criteria to achieve success. Address the new lab environment less highly skilled users with higher throughput requirements

33. Redefining the Way We Look at GC/MS Kit Software Hardware Productivity Solutions target routine GC/MS applications to offer value to the customer

34. EnviroLab Forms v 2.0High Throughput Quantification

35. EnviroLab Forms v 2.0 • An entirely new data processing and reporting system running as a layer over Xcalibur 1.4 • While targeted towards environmental laboratories and applications, it has been shown to be valuable for any laboratory performing high-throughput quantification • Adds a significant complement of capabilities that Xcalibur • Ion ratio calculations • Peak integration • Reporting • Integrated data review and reporting • No more batch reprocessing to re-generate reports of edited results • Fully automated while still allowing sophisticated manual review

36. Data Review

37. Report View

38. EnviroLab Forms v 2.0 • Brand new workflow oriented user interface • Substantially improved ion ratio review and editing support • Comprehensive set of textual and graphical reports • Fully automated, fully manual or any combination in between • Can be used as a high-throughput quantification engine for any application • Simply turn off or don’t use those options which aren’t relevant for a given user

39. Conclusion • The DSQ II system provides updates to hardware and software that make it easy to use • The Productivity Solution for method 8270 provides a method that has been validated across multiple DSQ II systems. • All of this allows a new operator to be productive immediately • The proven changes to the method allow an increased amount of productivity and throughput for the analyst and the laboratory.

40. Questions? Thank You