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Exp.6 Determination of total and direct bilirubin

Exp.6 Determination of total and direct bilirubin. Bilirubin : is the yellow breakdown product of normal haemoglobin. Bilirubin is excreted in bile and urine. Direct and Indirect bilirubin :. Clinical significance:. An increase in bilirubin conc. Is called jaundice.

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Exp.6 Determination of total and direct bilirubin

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  1. Exp.6Determination of total and direct bilirubin

  2. Bilirubin: is the yellow breakdown product of normal haemoglobin. • Bilirubin is excreted in bile and urine.

  3. Direct and Indirect bilirubin:

  4. Clinical significance: • An increase in bilirubin conc. Is called jaundice. • High level of direct type means that the bile is not being properly excreted. • High levels of indirect one indicate that too much haemoglobin is being damaged.

  5. Elevation of total bilirubin occur due to: • Excessive hemolysis of RBC • Liver diseases e.g. hepatitis &cirrhosis • Obstruction of biliary tract e.g. gall stones

  6. Principle: • Bilirubin + diazotized sulfanilic acid azobilirubin • The intensity of purple color is α to bilirubin conc. In serum • Bilirubinglucuronide +dizorgt.coloreddiazo compound(1min) • Unconjugated accelerates by methanol to form it • Total bilirubin is obtained after standing 5min colored formed measured at540nm

  7. Calculations: • Direct bilirubin(1min): • Abs(T)- Abs(test blank) x2.5 • Abs(bilirubin equivalent std) • Total bilirubin(5min): • Abs(T)- Abs(test blank) x5.0 • Abs(bilirubin equivalent std)

  8. Expected values in serum: • Direct ……………up to 0.5mg/dl • Total………………up to 1.0 mg/dl

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