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1. 818 Effect of smoking on gingival response to plaque accumulation CR Matthews1, V Joshi1, Marko de Jager2, Marcelo Aspiras2, PS Kumar1 1The Ohio State University, Columbus, OH, 2Philips Oral Healthcare, Snoqualmie, WA, USA Clinical Methods: Abstract: Results: Introduction: The effect of smoking on plaque-induced gingivitis has been previously reported. Although it is known that the severity of gingivitis is lower in smokers, it is not known if smoking affects the onset of clinical response to plaque accumulation. Objective: The purpose of this study was to compare clinical response to plaque accumulation between periodontally healthy current and never smokers during experimental gingivitis. Methods: 15 current and 15 non-smokers over age 18 without history of systemic disease, pregnancy, and recent or prophylactic antibiotic use were recruited. All subjects received a baseline examination and prophylaxis. Stents were fabricated to protect 3 adjacent teeth in 2 posterior sextants during brushing. Instructions were given to avoid mouthwash, flossing, or other hygiene aides in the area. Rustogi’s plaque index and Loe & Silness’s gingival index were recorded at baseline, 1, 2, 3, 4, 7, 14, and 21 days. Supragingival and subgingival plaque, and gingival crevicular fluid was also collected. At the conclusion of each visit, patients were scaled, polished, and flossed to ensure undisturbed plaque development for the following visit. Results: Smokers had a significant exposure to tobacco as compared to non-smokers (p<0.05). Plaque index was not significantly different between groups (p>0.05). The gingival index in both non-smokers and smokers showed a significant change from days 7 to 14 (p=0.0001, repeated measures ANOVA). Gingival crevicular fluid levels significantly increased in non-smokers from days 14 to 21 (p=0.01), while smokers showed no significant change. Smokers demonstrated moderate gingivitis (mean gingival index >1) at 4 days, while non-smokers reached this threshold at 14 days. Conclusions: Smokers demonstrated an earlier onset of moderate gingivitis as compared to non-smokers in response to similar amounts of plaque accumulation. Supported by a research grant from Philips OralHealthcare. Smokers Non-Smokers Plaque Index Gingival Index Introduction: • Bacterial biofilms form on the teeth within a few minutes after prophylaxis • Smokers have shown to have a less severe gingival inflammation when compared to non-smokers • Little evidence is known about the variation in onset of gingivitis between smokers and non-smokers Log Mean Cytokines Demographics: Purpose: Conclusions: To compare clinical response to plaque accumulation between periodontally healthy current and never smokers at 6 time periods over 21 days of experimental gingivitis. Smokers demonstrated an earlier onset of moderate gingivitis (GI < 1) as compared to non-smokers in response to similar amounts of plaque accumulation.