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Introduction Membrane Permeation System Experimental Section

This study investigates the hydrodynamic characteristics of an in-vitro membrane permeation system and its potential for calibration as a standardized in-vitro system. The permeation rate of drugs through various membranes is measured using a calibrated permeation cell, taking into consideration the effect of the diffusion boundary layer on permeation. Aqueous solubilities of benzoic acid in PEG 400 solutions are obtained, and diffusion coefficients are calculated using literature values and the Wilke equation. The experimental setup includes a membrane permeation system with a magnetic stirrer, donor and receptor reservoirs, and temperature control. Samples for analysis are prepared by diluting and filtering saturated solutions of benzoic acid.

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Introduction Membrane Permeation System Experimental Section

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  1. IntroductionMembrane Permeation SystemExperimental Section Presented By: Rich Dominiak Laura Kuczynski John Roszko

  2. Introduction • Mass transfer through various membranes is receiving increased attention • Drug delivery through polymer membranes and human or animal skin has become a challenging research area • In vitro setups are used to make permeation measurements for membrane-moderated controlled release of drugs • The effect of the diffusion boundary layer on permeation has not been studied

  3. Introduction • Permeation rate data can be easily distorted by the diffusion boundary layers on the membrane • The rate of drug permeation should be measured using a calibrated permeation cell • In this study • The hydrodynamic characteristics of the an in-vitro membrane permeation cell developed by Chien and Valia were investigated • Determine if it can be calibrated as a standardized in-vitro system

  4. Membrane Permeation System • Membrane surface area – 0.64 cm2 • Star head magnetic stirrer • Donor and receptor reservoirs • 3.5 mL capacity • Completley enclosed • Jacketed for temperature control

  5. Experimental Section • Objective: To obtain the aqueous solubilities of benzoic acid in various PEG 400 solutions • Saturated solutions of benzoic acid in water with 0-40% PEG 400 were prepared by placing an excess of benzoic acid in test tubes with the PEG solutions • They were placed in a shaking water bath for 24 hours

  6. Experimental Section • Filtered through 0.45 um Millipore filter • Diluted with solution medium that was used in the test tubes • Concentrations were analyzed using a UV/vis spectrophotometer • Viscosity was measured using and Ostwald capillary viscometer

  7. Experimental Section • Diffusion coefficients were calculated from literature values based on the diffusivity in pure water at 25 C • Wilke equation – 1.082 x 10-5 cm2/s • Thin benzoic acid disks were made by pouring fused benzoic acid into a metal mold positioned on a pill tile • Mounted to permeation cell

  8. Experimental Section • 3.5 mL of each solution (previously made) were pipetted into the receptor compartment while the donor compartment remained empty • At time intervals, 20 uL were withdrawn and diluted to 10 mL samples • These were analyzed with the UV spectrophotometer

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