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Investigating the Circular Genome of Smp131 Using Southern Hybridization Techniques

This study explores the genome structure of Smp131 to determine if it possesses a circular form. A detailed restriction map derived from the Smp131 sequence served as the foundation for the investigation. Southern hybridization was conducted with probes E1-3 (2.5 kb) and E5B1 (0.7 kb) to analyze fragments obtained from AvaI and EcoRV digests. The results from these hybridizations will provide insight into the genome configuration of Smp131 and confirm or refute its potential circularity based on probe hybridization patterns.

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Investigating the Circular Genome of Smp131 Using Southern Hybridization Techniques

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  1. (A) (B) cos site (1-19) E6 (33079) E1(2055) V12 (32366) E2(3516) V1 (3554) V2 (4511) E6-E1 V12-E6 Smp131 33525 bp E3 (11060) V11 (22201) V3 (11782) V10 (21817) EcoRV EcoRV V9(21793) V4 (12811) M M E4 (13311) V8 (18687) V5 (13915) V6 (14215) E5 (17935) V7 (17969) Figure S1 Strategy employed to test whether Smp131 has a circular form of genome. Lines: 1, restriction map deduced from the Smp131 sequence determined in this study; 2, fragments E1-3 (2.5 kb) and E5B1 (0.7 kb) used as probes for Southern hybridization; 3 and 4, 4.7-kb AvaI fragment (A1) and 4.7-kb EcoRV fragment (B5), respectively, that would hybridize to probes EI-3 and E5BI should the genome be circular. (B) Southern hybridization of AvaI and EcoRV digests from Smp131 genome using E1-3 and E5B1 separately as probes.

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