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Understanding Positive and Negative Peak Medians in Flow Cytometry Analysis

Learn about the differences between positive and negative peak medians, as well as the W=2 x rSD (robust standard deviation) for improved resolution sensitivity in flow cytometry. Explore insights from Dr. Holden Maecker on using various fluorochromes and stain index.

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Understanding Positive and Negative Peak Medians in Flow Cytometry Analysis

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  1. W1 W2 Where D = difference between positive and negative peak medians, and W = 2 x rSD (robust standard deviation) “Bright” = good resolution sensitivity D Holden Maecker, Ph.D.

  2. Various fluorochromes-stain index

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