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Sampling Methods

Sampling Methods. Sampling. Purpose Detect and quantify Identify sources/amplification Assess potential virulence Assess effectiveness of controls. Cladosporium. Steps in Sampling. Selection of a laboratory Selection of analyts Selection of methods Sensitivity of method

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Sampling Methods

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  1. Sampling Methods

  2. Sampling • Purpose • Detect and quantify • Identify sources/amplification • Assess potential virulence • Assess effectiveness of controls

  3. Cladosporium

  4. Steps in Sampling • Selection of a laboratory • Selection of analyts • Selection of methods • Sensitivity of method • Field sampling QC • Reporting and lab support

  5. Approaches to Sampling • Qualitative - Type • Quantitative - Concentration

  6. Overview of Microbial Sample Collection Methods(Liquid Impinger)

  7. Overview of Microbial Sample Collection Methods (Fungal Spore Sampling & Spore Traps)

  8. Overview of Microbial Sample Collection Methods(Impactor Samplers)

  9. Overview of Microbial Sample Collection Methods(Sedimentation Settling Plates)

  10. Overview of Microbial Sample Collection Methods(Airborne Particulates & Bulk Samples)

  11. Overview of Microbial Sample Collection Methods(Dust & Tape Lifts)

  12. Tape Lift Sampling

  13. Overview of Microbial SampleCollection Methods(Swab/Wipe & Surface Contact Plate)

  14. Overview of Microbial Sample Collection Methods(Water Sample & Endotoxins in air)

  15. Overview of Microbial Sample Collection Methods(Mycotoxins in Air)

  16. Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1,3-D-Glucans in Air)

  17. Overview of Microbial Sample Collection Methods(MVOC’s in Air & β-1,3-D-Glucans in Air)

  18. Microbial By Products(Ergosterol in Air) Notes: *Can also analyze for in bulk, surface and water samples. CFU-colony forming units M3-cubic metres of air Mg-milligrams Ng-nanograms Ppm-parts per million Ml-milliliter G-gram **References used to general Table 1 above are listed below 1-8

  19. environmentaleducation.com

  20. Air Flow Valve Biosis Rotameter Pump Plate Sampling

  21. Plate Sampling

  22. Stacked Plate Samplers

  23. Impinger Sampling • Impingement • Liquid media

  24. Impinger Sampling • Types of Impingers • AGI-30 • AGI-4

  25. Impinger Sampling

  26. Air Sampling • Types of Filtration • Membrane filter cassette

  27. Passive Sampling • Deposition • Gravity on agar

  28. Passive Sampling • Types of gravitational • Settling plates

  29. Air Sampling (Spore Trap) • Collects “everything” • Viable,non-viable,pollen, • Unable to differentiate

  30. Carpet Sampling • Collect spores in the carpet pile using air pump • Identifies type of mold • Looking for uncommon types • Provides historical information

  31. Carpet Sampling

  32. Data Interpretation • Concentration inside versus outside. • Mold types inside versus outside. • Presence or absence of a specific mold. • Location of unknown source. • Change in concentration following remediation. • Is a “snap shot” in time.

  33. Stachybotris

  34. Aspergillus

  35. environmentaleducation.com

  36. References

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